Anti-PRRG4 antibody (ab121298)

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Overview

  • Product name

    Anti-PRRG4 antibody

  • Description

    Rabbit polyclonal to PRRG4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to amino acids 144-226 of Human PRRG4 (Q9BZD6).

  • Positive control

    • RT4 and Human Liver lysates; Human duodenum tissue; U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab121298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Tissue specificity

    Expressed in lung, liver, kidney, pancreas and placenta.

  • Sequence similarities

    Contains 1 Gla (gamma-carboxy-glutamate) domain.

  • Post-translational
    modifications

    Gla residues are produced after subsequent post-translational modifications of glutamate by a vitamin K-dependent gamma-carboxylase.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 9930111I18Rik antibody
      • MGC117538 antibody
      • Proline-rich gamma-carboxyglutamic acid protein 4 antibody
      • Proline-rich Gla protein 4 antibody
      • PRRG 4 antibody
      • PRRG4 antibody
      • TMG4 antibody
      • TMG4_HUMAN antibody
      • Transmembrane gamma-carboxyglutamic acid protein 4 antibody
      see all

    Images

    • Western blot - Anti-PRRG4 antibody (ab121298)
      Western blot - Anti-PRRG4 antibody (ab121298)
      All lanes : Anti-PRRG4 antibody (ab121298) at 1/250 dilution

      Lane 1 : RT4 cell lysate.
      Lane 2 : Human Plasma
      Lane 3 : U-251 MG cell lysate.
      Lane 4 : Human Liver lysate.
      Lane 5 : Human Tonsil lysate.

      Developed using the ECL technique.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRG4 antibody (ab121298)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRG4 antibody (ab121298)
      ab121198at 1/350 dilution staining PRRG4 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-PRRG4 antibody (ab121298)
      Immunocytochemistry/ Immunofluorescence - Anti-PRRG4 antibody (ab121298)
      ab121298, at 4 µg/ml, staining PRRG4 (green) in PFA-fixed, Triton-permeabilized Human U-2 OS cells by Immunofluorescence.

    References

    ab121298 has not yet been referenced specifically in any publications.

    Publishing research using ab121298? Please let us know so that we can cite the reference in this datasheet.

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