- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PRRG4 antibody
See all PRRG4 primary antibodies -
DescriptionRabbit polyclonal to PRRG4
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to amino acids 144-226 of Human PRRG4 (Q9BZD6).
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Positive control
- RT4 and Human Liver lysates; Human duodenum tissue; U-2 OS cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab121298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Tissue specificityExpressed in lung, liver, kidney, pancreas and placenta.
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Sequence similaritiesContains 1 Gla (gamma-carboxy-glutamate) domain.
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Post-translational
modificationsGla residues are produced after subsequent post-translational modifications of glutamate by a vitamin K-dependent gamma-carboxylase. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 79056 Human
- Omim: 611690 Human
- SwissProt: Q9BZD6 Human
- Unigene: 471695 Human
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Alternative names
- 9930111I18Rik antibody
- MGC117538 antibody
- Proline-rich gamma-carboxyglutamic acid protein 4 antibody
see all
Images
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Western blot - Anti-PRRG4 antibody (ab121298)All lanes : Anti-PRRG4 antibody (ab121298) at 1/250 dilution
Lane 1 : RT4 cell lysate.
Lane 2 : Human Plasma
Lane 3 : U-251 MG cell lysate.
Lane 4 : Human Liver lysate.
Lane 5 : Human Tonsil lysate.
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRG4 antibody (ab121298)ab121198at 1/350 dilution staining PRRG4 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-PRRG4 antibody (ab121298)ab121298, at 4 µg/ml, staining PRRG4 (green) in PFA-fixed, Triton-permeabilized Human U-2 OS cells by Immunofluorescence.
Protocols
References
ab121298 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"