Recombinant Anti-Trypsin antibody [EPR19497] (ab200996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19497] to Trypsin
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Trypsin antibody [EPR19497]
See all Trypsin primary antibodies -
Description
Rabbit monoclonal [EPR19497] to Trypsin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human PRSS1 fragment recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19497 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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Notes |
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IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5644 Human
- Entrez Gene: 5645 Human
- Entrez Gene: 5646 Human
- Omim: 276000 Human
- Omim: 601564 Human
- Omim: 613578 Human
- SwissProt: P07477 Human
- SwissProt: P07478 Human
see all -
Alternative names
- Alpha-trypsin chain 2 antibody
- Anionic trypsinogen antibody
- Beta trypsin antibody
see all
Images
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All lanes : Anti-Trypsin antibody [EPR19497] (ab200996) at 1/1000 dilution
Lane 1 : Human PRSS1 fragment recombinant protein
Lane 2 : Human TRY3 full length recombinant protein
Lane 3 : Human TRY6 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2 and 3: 3 minutes.
Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag®. These three recombinant proteins were made in-house.
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Anti-Trypsin antibody [EPR19497] (ab200996) at 1/1000 dilution + Human TRY2 full length recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag. It was made in-house.
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All lanes : Anti-Trypsin antibody [EPR19497] (ab200996) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lane 4 : Human fetal kidney tissue lysate
Lane 5 : Human fetal spleen tissue lysate
Lane 6 : Human fetal lung tissue lysate
Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID: 3011602, 7945238, 24722290).
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab200996 has been referenced in 2 publications.
- Ye D et al. Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway. Int J Biol Sci 17:957-971 (2021). PubMed: 33867821
- Chen X et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). PubMed: 28858244