RecombinantRabMAb

Recombinant Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)

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Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
  • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
  • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
  • Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19497] to PRSS1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PRSS1 antibody [EPR19497] - BSA and Azide free
    See all PRSS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19497] to PRSS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251325 is the carrier-free version of ab200996. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251325 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Recombinant fragment
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.

  • Involvement in disease

    Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alpha-trypsin chain 2 antibody
      • Beta-trypsin antibody
      • Cationic trypsinogen antibody
      • Digestive zymogen antibody
      • Nonfunctional trypsin 1 antibody
      • Prss1 antibody
      • Serine protease 1 antibody
      • TCR V beta 4.1 antibody
      • TRP1 antibody
      • TRY1 antibody
      • TRY1_HUMAN antibody
      • TRY4 antibody
      • TRYP1 antibody
      • Trypsin I antibody
      • Trypsinogen 1 antibody
      • Trypsinogen A antibody
      see all

    Images

    • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution

      Lane 1 : Human PRSS1 fragment recombinant protein
      Lane 2 : Human TRY3 full length recombinant protein
      Lane 3 : Human TRY6 full length recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 27 kDa



      This data was developed using ab200996, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 1 second; Lanes 2-3: 3 minutes.

      Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag®. These three recombinant proteins were made in-house.

    • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution + Human TRY2 full length recombinant protein at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 27 kDa


      Exposure time: 3 minutes


      This data was developed using ab200996, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag. It was made in-house.

    • Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Western blot - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/5000 dilution

      Lane 1 : Human pancreas lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : Human fetal heart tissue lysate
      Lane 4 : Human fetal kidney tissue lysate
      Lane 5 : Human fetal spleen tissue lysate
      Lane 6 : Human fetal lung tissue lysate
      Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
      Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
      Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 27 kDa
      Observed band size: 27 kDa


      Exposure time: 3 minutes


      This data was developed using ab200996, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID: 3011602, 7945238, 24722290).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      This data was developed using ab200996, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      This data was developed using ab200996, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)
      Anti-PRSS1 antibody [EPR19497] - BSA and Azide free (ab251325)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251325? Please let us know so that we can cite the reference in this datasheet.

    ab251325 has not yet been referenced specifically in any publications.

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