Overview

  • Product name

    Anti-PRSS35 antibody
  • Description

    Rabbit polyclonal to PRSS35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-243 of Human PRSS35 (Q8N3Z0).

  • Positive control

    • PC3, SK N SH and Mouse testis whole cell lysates; Human U251 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Relevance

    PRSS35, although related to the peptidase S1 family, lacks the conserved active Ser residue in position 346 which is replaced by a Thr, suggesting that it has no protease activity. This Ser to Thr substitution may play an important role in determining a unique pattern of substrate specificity for PRSS35. Alternatively, this substitution may yield an inactive protease that binds to specific substrates, thus protecting them from proteolysis. The Mouse Prss35 gene is selectively expressed in the ovary and it's expression prior to ovulation is, in part, regulated by progesterone. PRSS35 may be involved in ovulation and the formation and regression of the corpus luteum.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • 6030424L22Rik antibody
    • C6orf158 antibody
    • Inactive serine protease 35 antibody
    • MGC46520 antibody
    • P3D9 antibody
    • protease, serine, 35 antibody
    • PRSS 35 antibody
    see all

Images

  • Anti-PRSS35 antibody (ab151970) at 1/1000 dilution + Mouse testis whole cell lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • All lanes : Anti-PRSS35 antibody (ab151970) at 1/1000 dilution

    Lane 1 : PC3 whole cell lysate
    Lane 2 : SK N SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human U251 xenograft tissue labeling PRSS35 with ab151970 at 1/500 dilution.

References

ab151970 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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