Anti-PRSS35 antibody (ab151970)
Key features and details
- Rabbit polyclonal to PRSS35
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PRSS35 antibody -
Description
Rabbit polyclonal to PRSS35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-243 of Human PRSS35 (Q8N3Z0).
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Positive control
- PC3, SK N SH and Mouse testis whole cell lysates; Human U251 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Relevance
PRSS35, although related to the peptidase S1 family, lacks the conserved active Ser residue in position 346 which is replaced by a Thr, suggesting that it has no protease activity. This Ser to Thr substitution may play an important role in determining a unique pattern of substrate specificity for PRSS35. Alternatively, this substitution may yield an inactive protease that binds to specific substrates, thus protecting them from proteolysis. The Mouse Prss35 gene is selectively expressed in the ovary and it's expression prior to ovulation is, in part, regulated by progesterone. PRSS35 may be involved in ovulation and the formation and regression of the corpus luteum. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 167681 Human
- Entrez Gene: 244954 Mouse
- SwissProt: Q8N3Z0 Human
- SwissProt: Q8C0F9 Mouse
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Alternative names
- 6030424L22Rik antibody
- C6orf158 antibody
- Inactive serine protease 35 antibody
see all
Images
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Anti-PRSS35 antibody (ab151970) at 1/1000 dilution + Mouse testis whole cell lysate at 50 µg
Predicted band size: 47 kDa
10% SDS PAGE -
All lanes : Anti-PRSS35 antibody (ab151970) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : SK N SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human U251 xenograft tissue labeling PRSS35 with ab151970 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151970 has been referenced in 1 publication.
- Van Hove L et al. Fibrotic enzymes modulate wound-induced skin tumorigenesis. EMBO Rep 22:e51573 (2021). PubMed: 33780134