Overview

  • Product name

  • Description

    Rabbit polyclonal to PRUNE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PRUNE aa 1-168.
    Database link: Q86TP1

  • Positive control

    • WB: HepG2, MCF7 and Jurkat whole cell lysate (ab7899). IHC-P: Human small intestine and lung tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
IP 1/200 - 1/2000.
IHC-P 1/20 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
  • Tissue specificity

    Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
  • Sequence similarities

    Belongs to the PPase class C family. Prune subfamily.
  • Cellular localization

    Cytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed.
  • Information by UniProt
  • Database links

  • Alternative names

    • DRES-17 antibody
    • DRES17 antibody
    • Drosophila-related expressed sequence 17 antibody
    • hPrune antibody
    • HTcD37 antibody
    • Protein prune homolog antibody
    • PRUNE antibody
    • Prune homolog antibody
    • PRUNE like protein antibody
    • PRUNE_HUMAN antibody
    • TcD37 homolog antibody
    see all

Images

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PRUNE (green) using ab237518 at 1/135 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

  • Paraffin-embedded human lung tissue stained for PRUNE using ab237518 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • PRUNE was immunoprecipitated from 500 μg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.  For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).

    Lane 1: Rabbit control IgG instead of ab237518 in HepG2 whole cell lysate.
    Lane 2: ab237518 IP in HepG2 whole cell lysate
    Lane 3: HepG2 whole cell lysate 10 μg (Input)

  • Paraffin-embedded human small intestine tissue stained for PRUNE using ab237518 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Recombinant Mouse CTLA4 protein (Tagged) (ab235718) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 50 kDa

References

ab237518 has not yet been referenced specifically in any publications.

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