Key features and details
- Rabbit polyclonal to PRUNE
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRUNE antibody
See all PRUNE primary antibodies
DescriptionRabbit polyclonal to PRUNE
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PRUNE aa 1-168.
Database link: Q86TP1
- WB: HepG2, MCF7 and Jurkat whole cell lysate (ab7899). IHC-P: Human small intestine and lung tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionPhosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
Tissue specificityUbiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
Sequence similaritiesBelongs to the PPase class C family. Prune subfamily.
Cellular localizationCytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed.
- Information by UniProt
- DRES-17 antibody
- DRES17 antibody
- Drosophila-related expressed sequence 17 antibody
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PRUNE (green) using ab237518 at 1/135 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Paraffin-embedded human lung tissue stained for PRUNE using ab237518 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
PRUNE was immunoprecipitated from 500 μg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 1: Rabbit control IgG instead of ab237518 in HepG2 whole cell lysate.
Lane 2: ab237518 IP in HepG2 whole cell lysate
Lane 3: HepG2 whole cell lysate 10 μg (Input)
Paraffin-embedded human small intestine tissue stained for PRUNE using ab237518 at 1/100 dilution in immunohistochemical analysis.
All lanes : Recombinant Mouse CTLA4 protein (Tagged) (ab235718) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237518 has not yet been referenced specifically in any publications.