Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab106834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 27 kDa).

Target

Images

  • Anti-PRX-1 antibody (ab106834) at 1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 27 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 hour

References

ab106834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Zebrafish Embryo (wholemount)
Specification
wholemount

Abcam user community

Verified customer

Submitted Dec 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Femur)
Antigen retrieval step
Other
Permeabilization
No
Specification
Femur
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Jul 03 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab106834 in mouse. This code will give you 1free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up