Recombinant Anti-PRX antibody [EPR24150-36] (ab259953)

Product name

Anti-PRX antibody [EPR24150-36]
See all PRX primary antibodies
Description

Rabbit monoclonal [EPR24150-36] to PRX
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC,IP or WB
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human PRX aa 700-900. The exact sequence is proprietary.
Database link: Q9BXM0
Positive control
- IHC-P: Human lung, cerebrum and skeletal muscle tissue; Mouse lung, cerebrum and skeletal muscle tissue; Rat lung and skeletal muscle tissue. IHC-Fr: Rat sciatic nerve and lung tissue; Mouse sciatic nerve and lung tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24150-36
Isotype

IgG
Research areas

Alternative Versions
- Anti-PRX antibody [EPR24150-36] - BSA and Azide free (ab278083)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Our Abpromise guarantee covers the use of ab259953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-Fr		1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC,IP or WB.

Function

Seems to be required for maintenance of peripheral nerve myelin sheath. May have a role in axon-glial interactions, possibly by interacting with the cytoplasmic domains of integral membrane proteins such as myelin-associated glycoprotein in the periaxonal regions of the Schwann cell plasma membrane. May have a role in the early phases of myelin deposition.
Tissue specificity

Isoform 1 and isoform 2 are found in sciatic nerve and Schwann cells.
Involvement in disease

Defects in PRX are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
Sequence similarities

Belongs to the periaxin family.
Contains 1 PDZ (DHR) domain.
Domain

Has a remarkable domain of repetitive pentameric units sometimes followed by a tripeptide spacer, it may separate two functional basic and acidic domains.
The Arg/Lys-rich basic domain functions as a tripartite nuclear localization signal.
Cellular localization

Cytoplasm; Nucleus and Cell membrane. Associated with plasma membrane during myelination.
Target information above from: UniProt accession Q9BXM0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 57716 Human
- Entrez Gene: 19153 Mouse
- Entrez Gene: 78960 Rat
- Omim: 605725 Human
- SwissProt: Q9BXM0 Human
- SwissProt: O55103 Mouse
- SwissProt: Q63425 Rat
- Unigene: 205457 Human
Alternative names
- CMT4F antibody
- KIAA1620 antibody
- Periaxin antibody
- PRAX_HUMAN antibody
- Prx antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PRX with ab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on the capillaries of human lung. The section was incubated with ab259953 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^®Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on the capillaries of mouse lung (PMID: 29968755). The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling PRX with ab259953 at 1/100 (4.8 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^®Polymer Refine Detection). Positive staining on the capillaries of human cerebrum (PMID: 29968755). The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve tissue labeling PRX with ab259953 at 1/250 (1.92 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on the nerve fibers of human skeletal muscle. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling PRX with ab259953 at 1/100 (4.8 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on the nerve fibers of mouse skeletal muscle. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve tissue labeling PRX with ab259953 at 1/250 (1.92 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on the capillaries of rat lung. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on the nerve fibers of rat skeletal muscle. The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRX antibody [EPR24150-36] (ab259953)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PRX with abab259953 at 1/2000 (0.24 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Negative control: no staining on the capillaries of mouse cerebrum (PMID:29968755). The section was incubated with ab259953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-PRX antibody [EPR24150-36] (ab259953)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab259953? Please let us know so that we can cite the reference in this datasheet.

ab259953 has not yet been referenced specifically in any publications.

Recombinant Anti-PRX antibody [EPR24150-36] (ab259953)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (0)

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