• Product name
    Anti-PSAP antibody [EPR10784(B)]
    See all PSAP primary antibodies
  • Description
    Rabbit monoclonal [EPR10784(B)] to PSAP
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PSAP (UniProt P07602).

  • Positive control
    • 293T, HepG2, A431, HeLa cell lysate. Human colon tissue. Human transitional call carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab166910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins).
      Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC and galactosylceramide by beta-galactosylceramidase (EC Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.
      Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC, GM1 gangliosides by beta-galactosidase (EC and globotriaosylceramide by alpha-galactosidase A (EC Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.
      Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC
    • Involvement in disease
      Defects in PSAP are the cause of combined saposin deficiency (CSAPD) [MIM:611721]; also known as prosaposin deficiency. CSAPD is due to absence of all saposins, leading to a fatal storage disorder with hepatosplenomegaly and severe neurological involvement.
      Defects in PSAP saposin-B region are the cause of leukodystrophy metachromatic due to saposin-B deficiency (MLD-SAPB) [MIM:249900]. MLD-SAPB is an atypical form of metachromatic leukodystrophy. It is characterized by tissue accumulation of cerebroside-3-sulfate, demyelination, periventricular white matter abnormalities, peripheral neuropathy. Additional neurological features include dysarthria, ataxic gait, psychomotr regression, seizures, cognitive decline and spastic quadriparesis.
      Defects in PSAP saposin-C region are the cause of atypical Gaucher disease (AGD) [MIM:610539]. Affected individuals have marked glucosylceramide accumulation in the spleen without having a deficiency of glucosylceramide-beta glucosidase characteristic of classic Gaucher disease, a lysosomal storage disorder.
      Defects in PSAP saposin-A region are the cause of atypical Krabbe disease (AKRD) [MIM:611722]. AKRD is a disorder of galactosylceramide metabolism. AKRD features include progressive encephalopathy and abnormal myelination in the cerebral white matter resembling Krabbe disease.
      Note=Defects in PSAP saposin-D region are found in a variant of Tay-Sachs disease (GM2-gangliosidosis).
    • Sequence similarities
      Contains 2 saposin A-type domains.
      Contains 4 saposin B-type domains.
    • Post-translational
      This precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins.
      N-linked glycans show a high degree of microheterogeneity.
      The one residue extended Saposin-B-Val is only found in 5% of the chains.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • A1 activator antibody
      • Cerebroside sulfate activator antibody
      • Co-beta-glucosidase antibody
      • Component C antibody
      • CSAct antibody
      • Dispersin antibody
      • GLBA antibody
      • Glucosylceramidase activator antibody
      • Proactivator polypeptide antibody
      • Proactivator polypeptide precursor antibody
      • Prosaposin (sphingolipid activator protein 1) antibody
      • prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody
      • Prosaposin antibody
      • Protein A antibody
      • Protein C antibody
      • PSAP antibody
      • SAP 1 antibody
      • SAP 2 antibody
      • SAP-1 antibody
      • SAP-2 antibody
      • SAP_HUMAN antibody
      • SAP1 antibody
      • Saposin A antibody
      • Saposin B antibody
      • Saposin B Val antibody
      • Saposin C antibody
      • Saposin D antibody
      • Saposin-D antibody
      • Saposins antibody
      • Sgp1 antibody
      • Sphingolipid activator protein 1 antibody
      • Sphingolipid activator protein 2 antibody
      • Sulfated glycoprotein 1 antibody
      • Sulfatide/GM1 activator antibody
      see all


    • All lanes : Anti-PSAP antibody [EPR10784(B)] (ab166910) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PSAP with ab166910 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma labeling PSAP with ab166910 at 1/50 dilution.


    This product has been referenced in:
    • Oldoni F  et al. Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans. Arterioscler Thromb Vasc Biol 38:1440-1453 (2018). Read more (PubMed: 29853565) »
    • Lu Q  et al. A novel probe to assess cytosolic entry of exogenous proteins. Nat Commun 9:3104 (2018). Read more (PubMed: 30082832) »
    See all 2 Publications for this product

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