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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-PSAP antibody [EPR10784(B)] (ab166910)

  • Datasheet
  • SDS
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Western blot - Anti-PSAP antibody [EPR10784(B)] (ab166910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSAP antibody [EPR10784(B)] (ab166910)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSAP antibody [EPR10784(B)] (ab166910)
  • Anti-PSAP antibody [EPR10784(B)] (ab166910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10784(B)] to PSAP
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-PSAP antibody [EPR10784(B)]
    See all PSAP primary antibodies
  • Description

    Rabbit monoclonal [EPR10784(B)] to PSAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human PSAP.
    Database link: P07602

  • Positive control

    • 293T, HepG2, A431, HeLa cell lysate. Human colon tissue. Human transitional call carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10784(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-PSAP antibody [EPR10784(B)] - BSA and Azide free (ab249391)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human PSAP protein (ab167924)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins).
      Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.
      Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.
      Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).
    • Involvement in disease

      Defects in PSAP are the cause of combined saposin deficiency (CSAPD) [MIM:611721]; also known as prosaposin deficiency. CSAPD is due to absence of all saposins, leading to a fatal storage disorder with hepatosplenomegaly and severe neurological involvement.
      Defects in PSAP saposin-B region are the cause of leukodystrophy metachromatic due to saposin-B deficiency (MLD-SAPB) [MIM:249900]. MLD-SAPB is an atypical form of metachromatic leukodystrophy. It is characterized by tissue accumulation of cerebroside-3-sulfate, demyelination, periventricular white matter abnormalities, peripheral neuropathy. Additional neurological features include dysarthria, ataxic gait, psychomotr regression, seizures, cognitive decline and spastic quadriparesis.
      Defects in PSAP saposin-C region are the cause of atypical Gaucher disease (AGD) [MIM:610539]. Affected individuals have marked glucosylceramide accumulation in the spleen without having a deficiency of glucosylceramide-beta glucosidase characteristic of classic Gaucher disease, a lysosomal storage disorder.
      Defects in PSAP saposin-A region are the cause of atypical Krabbe disease (AKRD) [MIM:611722]. AKRD is a disorder of galactosylceramide metabolism. AKRD features include progressive encephalopathy and abnormal myelination in the cerebral white matter resembling Krabbe disease.
      Note=Defects in PSAP saposin-D region are found in a variant of Tay-Sachs disease (GM2-gangliosidosis).
    • Sequence similarities

      Contains 2 saposin A-type domains.
      Contains 4 saposin B-type domains.
    • Post-translational
      modifications

      This precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins.
      N-linked glycans show a high degree of microheterogeneity.
      The one residue extended Saposin-B-Val is only found in 5% of the chains.
    • Cellular localization

      Lysosome.
    • Target information above from: UniProt accession P07602 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5660 Human
      • Omim: 176801 Human
      • SwissProt: P07602 Human
      • Unigene: 523004 Human
      • Alternative names

        • A1 activator antibody
        • Cerebroside sulfate activator antibody
        • Co-beta-glucosidase antibody
        • Component C antibody
        • CSAct antibody
        • Dispersin antibody
        • GLBA antibody
        • Glucosylceramidase activator antibody
        • Proactivator polypeptide antibody
        • Proactivator polypeptide precursor antibody
        • Prosaposin (sphingolipid activator protein 1) antibody
        • prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody
        • Prosaposin antibody
        • Protein A antibody
        • Protein C antibody
        • PSAP antibody
        • SAP 1 antibody
        • SAP 2 antibody
        • SAP-1 antibody
        • SAP-2 antibody
        • SAP_HUMAN antibody
        • SAP1 antibody
        • Saposin A antibody
        • Saposin B antibody
        • Saposin B Val antibody
        • Saposin C antibody
        • Saposin D antibody
        • Saposin-D antibody
        • Saposins antibody
        • Sgp1 antibody
        • Sphingolipid activator protein 1 antibody
        • Sphingolipid activator protein 2 antibody
        • Sulfated glycoprotein 1 antibody
        • Sulfatide/GM1 activator antibody
        see all

      Images

      • Western blot - Anti-PSAP antibody [EPR10784(B)] (ab166910)
        Western blot - Anti-PSAP antibody [EPR10784(B)] (ab166910)
        All lanes : Anti-PSAP antibody [EPR10784(B)] (ab166910) at 1/10000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : A431 cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 58 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSAP antibody [EPR10784(B)] (ab166910)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSAP antibody [EPR10784(B)] (ab166910)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PSAP with ab166910 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSAP antibody [EPR10784(B)] (ab166910)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSAP antibody [EPR10784(B)] (ab166910)

        Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma labeling PSAP with ab166910 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-PSAP antibody [EPR10784(B)] (ab166910)
        Anti-PSAP antibody [EPR10784(B)] (ab166910)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab166910? Please let us know so that we can cite the reference in this datasheet.

      ab166910 has been referenced in 2 publications.

      • Oldoni F  et al. Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans. Arterioscler Thromb Vasc Biol 38:1440-1453 (2018). PubMed: 29853565
      • Lu Q  et al. A novel probe to assess cytosolic entry of exogenous proteins. Nat Commun 9:3104 (2018). PubMed: 30082832

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