Overview

  • Product name
  • Description
    Rabbit polyclonal to PSCA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Bladder carcinoma and Prostate carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    PSCA (Prostate Stem Cell antigen) is a cell surface antigen, which is overexpressed in ~40% of primary prostate cancers and in as many as 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas. Antibody directed against PSCA inhibits tumorigenesis, slows tumor growth, prolongs survival and prevents metastasis in a preclinical zenograft model indicating that PSCA may have utility as a prognostic marker and/or therapeutic target in prostate cancer.
  • Cellular localization
    Cell membrane; Lipid-anchor, GPI-anchor.
  • Database links
  • Alternative names
    • PRO 232 antibody
    • PRO232 antibody
    • Prostate stem cell antigen antibody

Images

  • ab15168 staining PSCA in human breast carcinoma by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

References

This product has been referenced in:
  • Smith C  et al. Lack of association between the rs2294008 polymorphism in the prostate stem cell antigen gene and colorectal neoplasia: a case-control and immunohistochemical study. BMC Res Notes 5:371 (2012). IHC-P ; Human . Read more (PubMed: 22824379) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Antigen retrieval protocol: 1. Put tissue slides in 1X citrate buffer pH 6.0(we usually use 300ml container with 250ml buffer). 2. Microwave at high power for several minutes(one container for 2 and half minutes, two containers for 4 and half minutes.). To boil the buffer, please adjust the time with your own microwave. 3. Adjust microwave to lower power to keep buffer boiling for 10 minutes. 4. Leave the container inside microwave for at least 20 minutes. Let the buffer cold down to about 40 degree C. I hope this is helpful. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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