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  1. Link

    psca-antibody-ab202966.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-PSCA antibody (ab202966)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSCA antibody (ab202966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSCA antibody (ab202966)

Key features and details

  • Rabbit polyclonal to PSCA
  • Suitable for: IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human PSCA protein (ab159927)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PSCA antibody
    See all PSCA primary antibodies
  • Description

    Rabbit polyclonal to PSCA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human PSCA aa 5-55 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    LLALLMAGLALQPGTALLCYSCKAQVSNEDCLQVENCTQLGEQCWTARIR A


    Database link: O43653
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat testis and brain tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 0.01% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Stem Cells
    • Cancer Stem Cells
    • Intracellular

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PSCA protein (ab159927)

Applications

Our Abpromise guarantee covers the use of ab202966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

Target

  • Relevance

    PSCA (Prostate Stem Cell antigen) is a cell surface antigen, which is overexpressed in ~40% of primary prostate cancers and in as many as 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas. Antibody directed against PSCA inhibits tumorigenesis, slows tumor growth, prolongs survival and prevents metastasis in a preclinical zenograft model indicating that PSCA may have utility as a prognostic marker and/or therapeutic target in prostate cancer.
  • Cellular localization

    Cell membrane; Lipid-anchor, GPI-anchor.
  • Database links

    • Entrez Gene: 8000 Human
    • Entrez Gene: 680210 Rat
    • Omim: 602470 Human
    • SwissProt: O43653 Human
    • Unigene: 652235 Human
    • Unigene: 230062 Rat
    • Alternative names

      • PRO 232 antibody
      • PRO232 antibody
      • Prostate stem cell antigen antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSCA antibody (ab202966)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSCA antibody (ab202966)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat brain tissue labeling PSCA with ab202966 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSCA antibody (ab202966)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSCA antibody (ab202966)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat testis tissue labeling PSCA with ab202966 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab202966? Please let us know so that we can cite the reference in this datasheet.

    ab202966 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PSCA antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Eyes)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - Tween
    Specification
    Eyes
    Blocking step
    Gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.2%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 15 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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