Overview

  • Product name

  • Description

    Rabbit polyclonal to PSD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PSD3 aa 998-1048. The exact sequence is proprietary.
    Database link: Q9NYI0

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to PSD3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

20 µl/mg lysate

WB 1/1000. Predicted molecular weight: 116 kDa.

Target

  • Relevance

    PSD3 is a guanine nucleotide exchange factor (GEF) specific for ARF6-like protein. The gene is potential tumour suppressor or antagonising gene candidate for ovarian carcinoma.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity).
  • Database links

  • Alternative names

    • ADP ribosylation factor guanine nucleotide factor 6 antibody
    • DKFZp761K1423 antibody
    • EFA 6R antibody
    • EFA6R antibody
    • Epididymis tissue protein Li 20mP antibody
    • Exchange factor for ADP ribosylation factor guanine nucleotide factor 6 antibody
    • HCA 67 antibody
    • HCA67 antibody
    • Hepatocellular carcinoma associated antigen 67 antibody
    • KIAA0942 antibody
    • PH and SEC7 domain containing protein 3 antibody
    • Pleckstrin and Sec7 domain containing 3 antibody
    • Pleckstrin and Sec7 domain containing protein 3 antibody
    • Pleckstrin homology and SEC7 domain containing protein 3 antibody
    • PSD 3 antibody
    • PSD3 protein antibody
    see all

Images

  • All lanes : Anti-PSD3 antibody (ab241376) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 116 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • PSD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241376 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab241376 at 1/100 dilution.

    Lane 1: ab241376 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    Lysates prepared in NETN buffer.

References

ab241376 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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