Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)](https://www.abcam.com/ps/products/2/ab2723/Images/ab2723-352022-ab2723AP3930684IHCPRatretinal2ugDS.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)")
Key features and details
- Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Overview
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Product name
Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker
See all PSD95 primary antibodies -
Description
Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker -
Host species
Mouse -
Specificity
We do not guarantee IHC-P for mouse.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Zebrafish
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Immunogen
Recombinant full length protein corresponding to Rat PSD95.
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Positive control
- IHC-P: Rat retina tissue. WB: Rat and mouse hippocampus and forebrain tissue lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
6G6-1C9 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse. |
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| WB | (7) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
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| Notes |
|---|
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. We do not guarantee IHC-P for mouse. |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa). |
Target
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Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
Post-translational
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 100137840 Cow
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Entrez Gene: 405796 Zebrafish
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- SwissProt: Q6R005 Zebrafish
- Unigene: 27256 Mouse
see all -
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
IHC image of ab2723 staining in formalin-fixed, paraffin-embedded rat retinal tissue, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2723, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Secondary-only control image is shown as insert.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)](https://www.abcam.com/ps/products/2/ab2723/Images/ab2723-6251-anti-psd95-antibody-6g6-1c9-western-blot.jpg)
Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)All lanes : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 5 µg/ml
Lane 1 : Hippocampus (Rat) Tissue Lysate
Lane 2 : Forebrain (Rat) Tissue Lysate
Lane 3 : Hippocampus (Mouse) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
![Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)](https://www.abcam.com/ps/products/2/ab2723/Images/ab2723-263309-anti-psd95-antibody-6g6-1c9-western-blot.jpg)
Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)Image courtesy of an AbReview submitted by Grant CorbettLanes 1-6 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution
Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution (18 hours at 4°C)
Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (117)
ab2723 has been referenced in 117 publications.
- Lundgren JL et al. Proximity ligation assay reveals both pre- and postsynaptic localization of the APP-processing enzymes ADAM10 and BACE1 in rat and human adult brain. BMC Neurosci 21:6 (2020). PubMed: 32019490
- Post KL et al. Multi-model functionalization of disease-associated PTEN missense mutations identifies multiple molecular mechanisms underlying protein dysfunction. Nat Commun 11:2073 (2020). PubMed: 32350270
- Wu Q et al. SRC-1 Knockout Exerts No Effect on Amyloid ß Deposition in APP/PS1 Mice. Front Aging Neurosci 12:145 (2020). PubMed: 32625077
- Feng C et al. Calcium-Sensing Receptor Mediates ß-Amyloid-Induced Synaptic Formation Impairment and Cognitive Deficits via Regulation of Cytosolic Phospholipase A2/Prostaglandin E2 Metabolic Pathway. Front Aging Neurosci 12:144 (2020). PubMed: 32670047
- Gao BY et al. Modified constraint-induced movement therapy alters synaptic plasticity of rat contralateral hippocampus following middle cerebral artery occlusion. Neural Regen Res 15:1045-1057 (2020). PubMed: 31823884