• Product name
  • Description
    Goat polyclonal to PSD95
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from residues 1 - 100 of Mouse PSD95.

  • Positive control
    • This antibody gave a positive signal in WB using Mouse, Rat and Human brain tissue lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.025% Sodium azide
    Constituents: PBS, 1% BSA

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab12093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).

Abcam recommends using 3% milk as the blocking agent.

IHC-P Use a concentration of 10 µg/ml.


  • Function
    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • Tissue specificity
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Domain
    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • Post-translational
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • Cellular localization
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all


  • Lanes 1-4 : Anti-PSD95 antibody (ab12093) at 2 µg/ml (Blocked with 2% BSA)
    Lanes 5-8 : Anti-PSD95 antibody (ab12093) at 2 µg/ml (Blocked with 3% Milk)

    Lanes 1 & 5 : Human brain cerebral cortex tissue lysate - total protein (ab30061)
    Lanes 2 & 6 : Human brain tissue lysate - total protein
    Lanes 3 & 7 : Brain (Mouse) Tissue Lysate
    Lanes 4 & 8 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 110 kDa, 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin or 3% Milk before being incubated with ab12093 overnight at 4°C. Antibody binding was detected using an anti-goat antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • ab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm.
  • ab12093 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde and blocked with 10% donkey serum in  0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab12093 at 1/500 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-goat polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 expressed in exon, dendrites and particularly carrying in the dendritic spines

    See Abreview

  • All lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilution

    Lane 1 : rat cortical lysate
    Lane 2 : mouse cortical lysate
    Lanes 3-8 : BA21 human cortical lane lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Alexafluor 680

    Performed under reducing conditions.

    Predicted band size: 80 kDa

    See abreview for further details

    See Abreview

  • Lane 1 : Anti-PSD95 antibody (ab12093) at 0.3 µg/ml
    Lane 2 : Anti-PSD95 antibody (ab12093) at 1 µg/ml
    Lanes 3-4 : Anti-PSD95 antibody (ab12093) at 3 µg/ml

    Lanes 1-3 : 10µg mouse brain extract/lane
    Lane 4 : 10µg mouse brain extract/lane with Mouse PSD95 peptide (ab12419) at 1 µg/ml

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: ~105 kDa (why is the actual band size different from the predicted?)

    Exposure time: 10 seconds

    PSD95 (ab12093) western blot on mouse brain extract (10µg protein/lane), showing use of the immunising peptide (ab12419) as a control. The blot was exposed for 10 seconds to autoradiographic film.

    Lane 1 = 0.3µg ab12093
    Lane 2 = 1µg ab12093
    Lane 3 = 3µg ab12093
    Lane 4 = 3µg ab12093 + 1µg peptide
    Lane 5 = Ladder


This product has been referenced in:
  • Urraca N  et al. Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons. Mol Autism 9:6 (2018). WB . Read more (PubMed: 29423132) »
  • Wang J  et al. Epigenetic modulation of inflammation and synaptic plasticity promotes resilience against stress in mice. Nat Commun 9:477 (2018). IHC-P ; Mouse . Read more (PubMed: 29396460) »

See all 29 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Zebrafish Tissue sections (Retina, plexiform layers)
Yes - triton X
Retina, plexiform layers
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 05 2014

I am glad to read that most of the antibodies you ordered are giving good results. For the two that are not, could you please send some details of the samples and protocols and results? In particular, we would like to know the species, the time in PFA,...

Read More

Thank you for contacting us. I want to apologize for the delay as we arranged to add each peptide to our catalog.

The blocking peptides which you have requested are now available and can be located at the following links:


Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Brain)
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Jun 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cell (Primary Hippocampal neuron culture)
Primary Hippocampal neuron culture
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 16 2012

Immunohistochemistry free floating
Mouse Tissue sections (Coronal brain sections)
Coronal brain sections

Abcam user community

Verified customer

Submitted Oct 31 2011

Thank you for contacting us. We do not have anti GluR1 and GLuR2 antibodies that can be used for Zebrafish. We unfortunately do not have information of GluR1 and GLuR2 protein sequence of Zebrafish so I am sorry we can’t check the homology. How...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (BA21 human cortical tissue lysates)
Loading amount
20 µg
BA21 human cortical tissue lysates
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Martin Broadstock

Verified customer

Submitted Apr 01 2009

Thank you for your rapid feedback, this information indeed helps. Could you please consider submitting a review when you have some time and in return we will offer you 50 Abcam Loyalty points which you can use to redeem a variety of gifts (another 1...

Read More

Thank you for your e-mail, I am glad to hear you have solved your problem, would you mind letting me know what it was so I can close this enquiry? Thank you in advance,

1-10 of 12 Abreviews or Q&A


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