Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PSD95 antibody - Synaptic Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum and Medulla)
Specification
Cerebellum and Medulla
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Ab18258 (1:500) staining PSD95 in Immunohistochemistry paraffin-embedded Mouse Cerebellum and Medulla tissue.

This antibody gives a highly localised, mostly punctate positivity to the synaptic regions in the mouse brain.

Composite sagittal section image submitted: Upper image - of the cerebellum shows positivity in Glomeruli of the Granule cell layer, Purkinje cell somas (cell bodies) and their primary and secondary processes. Cerebellar white matter is negative. Lower image - of the medullary region of the spinal cord shows neurone positivity and a lighter positivity in the associated neuropil (grey matter) medullary axonal tracks (running vertically) appear negative.

Mr. Carl Hobbs

Verified customer

Submitted Mar 05 2010

Sign up