Western blot abreview for Anti-PSD95 antibody - Synaptic Marker

Excellent
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Marmoset (common) Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk in TBST
Dilution
1/350

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/12000

Detection

Detection method
Chemiluminescence
Bands
Specific: 80 kDa
Exposure
16 second(s)

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Submitted Apr 23 2014

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