Western blot abreview for Anti-PSD95 antibody

Average
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary culture hippocampus cell)
Gel Running Conditions
Non-reduced Denaturing (15% SDS-PAGE)
Loading amount
15 µg
Treatment
RIPA & 1% Protease inhibitor for 15min on ice
Specification
Primary culture hippocampus cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: ZSJQ IgG/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 85 kDa

Additional data

Additional Notes
Compared with other antibodies, the signal of this antibody is very weak. It is suggested to use traditional film method when detecting the signal.

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Submitted Oct 10 2018

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