Overview

  • Product name

    Anti-PSD95 antibody [C2D4]
    See all PSD95 primary antibodies
  • Description

    Rabbit monoclonal [C2D4] to PSD95
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PSD95 aa 11-24.
    Sequence:

    KYRYQDEDTPPLEH(c)


    Database link: P78352

  • Positive control

    • WB: Mouse brain extract; U-87 MG cell extract. IHC-P: Mouse cerebellum tissue; rat brain and cerebellum tissue. ICC/IF: SH-SY5Y and N2A cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab238513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 80 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Domain

    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • Post-translational
    modifications

    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • Cellular localization

    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Images

  • Paraffin-embedded rat brain tissue stained for PSD95 using ab238513 at 1/100 dilution in immunohistochemical analysis (40X lens).

  • SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for PSD95 (green) using ab238513 at 1/50 dilution in ICC/IF.

  • All lanes : Anti-PSD95 antibody [C2D4] (ab238513) at 1/500 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell extract

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1/10000 dilution

    Predicted band size: 80 kDa



    Blocking buffer: 3% non-fat dry milk in TBST.

  • Paraffin-embedded mouse cerebellum tissue stained for PSD95 using ab238513 at 1/100 dilution in immunohistochemical analysis (40X lens).

  • Paraffin-embedded rat cerebellum tissue stained for PSD95 using ab238513 at 1/100 dilution in immunohistochemical analysis (40X lens).

  • N2A cells stained for PSD95 (green) using ab238513 at 1/50 dilution in ICC/IF.

References

ab238513 has not yet been referenced specifically in any publications.

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