Key features and details
- Rabbit monoclonal [C2D4] to PSD95
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PSD95 antibody [C2D4]
See all PSD95 primary antibodies
DescriptionRabbit monoclonal [C2D4] to PSD95
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Mouse brain extract; U-87 MG cell extract. IHC-P: Mouse cerebellum tissue; rat brain and cerebellum tissue. ICC/IF: SH-SY5Y and N2A cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab238513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 80 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
DomainThe PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
- Information by UniProt
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
Paraffin-embedded rat brain tissue stained for PSD95 using ab238513 at 1/100 dilution in immunohistochemical analysis (40X lens).
SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for PSD95 (green) using ab238513 at 1/50 dilution in ICC/IF.
All lanes : Anti-PSD95 antibody [C2D4] (ab238513) at 1/500 dilution
Lane 1 : Mouse brain extract
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell extract
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1/10000 dilution
Predicted band size: 80 kDa
Blocking buffer: 3% non-fat dry milk in TBST.
Paraffin-embedded mouse cerebellum tissue stained for PSD95 using ab238513 at 1/100 dilution in immunohistochemical analysis (40X lens).
Paraffin-embedded rat cerebellum tissue stained for PSD95 using ab238513 at 1/100 dilution in immunohistochemical analysis (40X lens).
N2A (mouse) cells stained for PSD95 (green) using ab238513 at 1/50 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238513 has not yet been referenced specifically in any publications.