Key features and details
- Rabbit polyclonal to PSD95 - Synaptic Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human, Zebrafish, Cynomolgus monkey, Common marmoset
- Isotype: IgG
Product nameAnti-PSD95 antibody - Synaptic Marker
See all PSD95 primary antibodies
DescriptionRabbit polyclonal to PSD95 - Synaptic Marker
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish, Cynomolgus monkey, Common marmoset
Synthetic peptide corresponding to Mouse PSD95 aa 50-150 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
(Peptide available as
- WB: Mouse and rat brain lysates. ICC/IF: Zebrafish retina (inner plexiform layer) tissue, SH-SY5Y cell line.
Rabbit polyclonal to PSD95 - Synaptic Marker
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab18258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).
Abcam recommends using 3% milk as the blocking agent.
|IHC-P||Use at an assay dependent concentration.|
FunctionInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
DomainThe PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
- Information by UniProt
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
All lanes : Anti-PSD95 antibody - Synaptic Marker (ab18258) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 2 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 10 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
ab18258 (1/500) staining PSD95 in paraffin-embedded mouse cerebellum (top) and medulla (bottom) tissue, showing positive staining to the synaptic regions of the brain. Tissue was fixed in formaldehyde, blocking performed using 1% BSA (10 minutes/RT) and heat mediated antigen retrieval performed before staining. The secondary antibody (1/200) was goat anti rabbit IgG conjugated to Biotin. For further experimental details, please refer to abreview.
ab18258 (1/1000) staining PSD9 in paraffin-embedded rat cerebellum. Tissue was fixed in formaldehyde, blocking performed using 1% BSA (10 minutes/RT) and heat mediated antigen retrieval performed before staining. The secondary antibody (1/200) was goat anti rabbit IgG conjugated to Biotin. For further experimental details, please refer to abreview.
ab18258 staining PSD95 in SH-SY5Y cells. The cells were fixed with 100% methanol (5 min) at room temperature, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab18258 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
ab18258 staining PSD95 in zebrafish retina (inner plexiform layer) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Sodium citrate antigen retrieval was used. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
IHC image of PSD95 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab18258, 1 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab18258 staining PSD95 in human SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, blocked with 10% serum for 20 minutes at 24°C, then incubated with ab18258 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor® 488 conjugated donkey anti-rabbit polyclonal used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
All lanes : Anti-PSD95 antibody - Synaptic Marker (ab18258) at 1/350 dilution
All lanes : Marmoset (common) brain whole tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/12000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 16 seconds
ab18258 has been referenced in 175 publications.
- Hwang I & Ahn JY Dysregulation of Epigenetic Control Contributes to Schizophrenia-Like Behavior in Ebp1+/- Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32283721
- You MJ et al. Human umbilical cord-derived mesenchymal stem cells alleviate schizophrenia-relevant behaviors in amphetamine-sensitized mice by inhibiting neuroinflammation. Transl Psychiatry 10:123 (2020). PubMed: 32341334
- Nie PY et al. miR-132 Regulates PTSD-like Behaviors in Rats Following Single-Prolonged Stress Through Fragile X-Related Protein 1. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 32333305
- Kriebel M et al. Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity. Front Mol Neurosci 13:43 (2020). PubMed: 32265651
- Lee DS & Kim JE PDI-Mediated Reduction of Disulfide Bond on PSD95 Increases Spontaneous Seizure Activity by Regulating NR2A-PSD95 Interaction in Epileptic Rats Independent of S-Nitrosylation. Int J Mol Sci 21:N/A (2020). PubMed: 32197489