Product nameAnti-PSD95 (phospho S295) antibody [EP2615Y]
See all PSD95 primary antibodies
DescriptionRabbit monoclonal [EP2615Y] to PSD95 (phospho S295)
Tested applicationsSuitable for: WB, IP, ICCmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
A synthetic phospho-peptide corresponding to residues surrounding serine 295 of human PSD95
- Human brain tissue and rat brain lysates, untreated and treated with LP.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).|
FunctionInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
DomainThe PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
- Information by UniProt
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
All lanes : Anti-PSD95 (phospho S295) antibody [EP2615Y] (ab76108) at 1/20000 dilution
Lane 1 : human brain tissue lysate, treated with LP
Lane 2 : human brain tissue lysate untreated
Lane 3 : rat brain lysate, treated with LP
Lane 4 : rat brain lysate, untreated
Predicted band size: 95 kDa
Observed band size: 95 kDa
This product has been referenced in:
- Feng Y et al. Neuroprotective effects of resveratrol against traumatic brain injury in rats: Involvement of synaptic proteins and neuronal autophagy. Mol Med Rep 13:5248-54 (2016). Read more (PubMed: 27122047) »
- Zhu YC et al. Palmitoylation-dependent CDKL5-PSD-95 interaction regulates synaptic targeting of CDKL5 and dendritic spine development. Proc Natl Acad Sci U S A 110:9118-23 (2013). Read more (PubMed: 23671101) »