Overview

  • Product name
    Anti-PSD95 (phospho T19) antibody
    See all PSD95 primary antibodies
  • Description
    Rabbit polyclonal to PSD95 (phospho T19)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PSD95, phosphorylated at T19.

    Read Abcam's proprietary immunogen policy (Peptide available as ab17167.)

  • Positive control
    • mouse brain
  • General notes


    Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).

Target

  • Function
    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Domain
    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • Post-translational
    modifications
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • Cellular localization
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Images

  • Lanes 1 & 3 : ab16496 at 10 µg
    Lane 2 : ab16496

    Lane 1 : Mouse Brain
    Lane 2 : Mouse Brain with with modified blocking peptide at 1 µg/ml
    Lane 3 : Mouse Brain with with non-modified blocking peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    In WB, we recommend blocking in milk. Blocking with BSA has been seen to give high background with this antibody.

References

ab16496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% gel)
Sample
Mouse Tissue lysate - whole (mouse brain tissue)
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 17 2013

Answer

Thank you for contacting us.

Currently we only have one lot in stock of ab16496, which is GR91281-1. We are not planning on making a new lot until all10 vials of this lotin stockare sold.

We have not heard any complaints about this antibody since we've added it to the catalogue. The only trouble I see is your previous notification of the vial being crushed in shipping, but we do not have any quality issues.

I'm attaching the QC from the lot GR91281-1. You'll see lanes 4 - 6 were blocked with the phospho-peptide. The image caption is below:

Lanes 1 - 9 : Anti-PSD95 (phospho T19) antibody (ab16496) at 1 µg/ml (lot GR91281-1, available)
Lanes 10 - 12 : Anti-PSD95 (phospho T19) antibody (ab16496) at 1 µg/ml (lot 630291, sold out)

Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 2 : Forebrain (Mouse) Tissue Lysate (ab48634)
Lane 3 : Rat Forebrain Rat Tissue Lysate (ab65668)
Lane 4 : Brain (Mouse) Tissue Lysate (ab27253) with http://us.adminsite.com/index.html?datasheet=17167 at 1 µg/ml
Lane 5 : Forebrain (Mouse) Tissue Lysate (ab48634) with http://us.adminsite.com/index.html?datasheet=17167 at 1 µg/ml
Lane 6 : Rat Forebrain Rat Tissue Lysate (ab65668) with http://us.adminsite.com/index.html?datasheet=17167 at 1 µg/ml
Lane 7 : Brain (Mouse) Tissue Lysate (ab27253) with http://us.adminsite.com/index.html?datasheet=12419 at 1 µg/ml
Lane 8 : Forebrain (Mouse) Tissue Lysate (ab48634) with http://us.adminsite.com/index.html?datasheet=12419 at 1 µg/ml
Lane 9 : Rat Forebrain Rat Tissue Lysate (ab65668) with http://us.adminsite.com/index.html?datasheet=12419 at 1 µg/ml
Lane 10 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 11 : Forebrain (Mouse) Tissue Lysate (ab48634)
Lane 12 : Rat Forebrain Rat Tissue Lysate (ab65668)

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 100 kDa

Exposure time : 30 seconds



This antibody is covered under the Abpromise for detection of the phospho-protein in mouse in IHC-P and WB. If you have any difficulty with it in the tested species and applications, contact us within 6 months of purchase and we would be happy to replace or refund it.



I'm not sure if you need multple vials of the antibody or other products (secondaries, control peptide ab17167) but we're running a 4 for the price of 3 promotion for the rest of September. If you buy any 4 products, the least expensive one is free! Just mention the code 4FOR3-TB3ME on your order online or on the phone.



https://www.abcam.com/psd95-peptide-phospho-t19-ab17167.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

I am sorry for the delay in getting back to you.

I can now confirm that we do not unfortunately have any antibodies directed towards the N-terminal region of PSD95 which shares high homology with the drosophila dlg-A protein.

This means that the antibody most likely to react with the protein you are interested in is ab90426 as discussed in our previous emails. As the homology is not perfect, I cannot be sure that the antibody will work but it may be worth trying. If you would like to participate in the testing discount scheme previously discussed with this antibody then please do let me know and I will generatethediscount.

More information on the testing discount scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

I look forward to receiving your reply.

Read More

Answer

Thank you for getting back to me and I am sorry for the delay in getting back to you.

From the reference provided, it seems that the protein you wish to detect (dlgA) is produced from the gene dlgfrom which dlg1 isexpressed. The isoform E of dlg1 is designated as dlg-A and is the protein I think you want to detect. When taking this isoform and performing the same sequence comparison as I presented to you previously, it can again be seen that it is the C-terminal domain that shares the highest degree of homology with the mouse or human protein. The immunogen used to raise ab90426 again shares 8 of the 15 residues with the drosophila dlg-A protein and may therefore be able to detect the protein. I can offer you the testing discount for this antibody if you would like.

There are also certain regions within the N-terminal domain which matches well to the human protein, I am currently looking into if we have any antibodies which may be directed against this region specifically in order to see if I can find one that may be a better suggestion than ab90426. I will get back to you as soon as I can with this information.

I hope this information has been of help.

Read More

Answer

Thank you for contacting us and sorry for the delay in getting back to you.

I have looked intowhether theantibody you areinterested in, theanti-PSD95 (phospho T19) antibody (ab16496) would be likely to beable todetect the Drosophila protein and why this has been indicated on the datasheet of this product.

I am sorry to say that I think this informationwas added in error. I have not been able to find a sequence of theDrosophilaPSD95 protein and comparison of theMAGUK Drosophila protein Discs Large (otherwise known as Dlg, SwissProt reference http://www.uniprot.org/uniprot/P31007),it does notshare very high homology with the mouse orhuman protein (SwissProt reference Q62108 and P78352 respectively). The highest alignment is within the C-terminal domain, please refer the attachment of this email.

The alignment around the residue T19, from which the antibody ab16496 is raised is very poor and I would therefore not expect for the antibody to be able to detect the Drosophila protein. We do however have an antibody which is raised against the C-terminal end of the human protein, goat polyclonal ab90426. I have highlighted the immunogen used to raise this antibody in the attachment. As you can see, the alignment is not perfect, 5 of the 13 residues no not match, so I would not be sure of the antibody being able to detect the Dlg Drosophilaprotein. However if you would like to trythis antibodyI would be able to provide you with a testing discount for this product.

I am sorry that the information on the datasheet of ab16496 was misleading. I have now updated this information.

Please do let me know if you have any further questions and if you would like me to generated the discount code for ab90426.

I look forward to receiving your reply.

Read More

Answer

Thank you for contacting me yesterday and this morning.

I am sorry for the delay in getting back to you but as explained over the phone I am trying to ascertain the likelihood of the PSD95 antibody being able to detect the drosophila protein. This antibody has been raised against a peptide taken from within the first 100 residues of the mouse protein. I am currently trying to find a protein sequence of the Drosophila protein so that I can compare the region within the two proteins to see how likely it is that the antibody will also be able to detect the Drosophila protein. As we have several antibodies against this target, it may also mean that I can suggest other alternatives which may work better for your experiment. I will continue with this search and will get back to you as soon as I can with what I find.

In the meantime, I promised I would send you some information relating to the testing discount scheme. The way this scheme works is that if a particular species or application you wish to use is not listed on the datasheet of a product, you may be eligible for the testing discount scheme. This means you would purchase the antibody as normal, test it in your new species or application then share the results with us through an Abreview. Regardless of whether the results are positive or negative, you would then be eligible for a free primary antibody of your choice (or the value of the testing antibody off any product in our catalogue). The only constraint on this offer is that the antibody must be tested, the Abreview submitted and the free product claimed within a 4 month period.

More information on this offer can be accessed from the following link:

https://www.abcam.com/collaboratordiscount

I will get back toyou as soon as Ican once I have more informationon the Drosophila sequence. I am sorry for the delay and any inconvenience thisis causing you.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Feb 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal cord dorsal horn lysate)
Loading amount
15 µg
Specification
Spinal cord dorsal horn lysate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Richard D'Mello

Verified customer

Submitted Mar 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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