Anti-PSD95 (phospho T19) antibody (ab16496)

Overview

  • Product name
    Anti-PSD95 (phospho T19) antibody
    See all PSD95 primary antibodies
  • Description
    Rabbit polyclonal to PSD95 (phospho T19)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PSD95, phosphorylated at T19.

    (Peptide available as ab17167.)

  • Positive control
    • mouse brain
  • General notes


    Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).

Target

  • Function
    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • Domain
    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • Post-translational
    modifications
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • Cellular localization
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Images

  • Lanes 1 & 3 : ab16496 at 10 µg
    Lane 2 : ab16496

    Lane 1 : Mouse Brain
    Lane 2 : Mouse Brain with with modified blocking peptide at 1 µg/ml
    Lane 3 : Mouse Brain with with non-modified blocking peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 minutes


    In WB, we recommend blocking in milk. Blocking with BSA has been seen to give high background with this antibody.

References

ab16496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% gel)
Sample
Mouse Tissue lysate - whole (mouse brain tissue)
Specification
mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2013

Thank you for contacting us.

Currently we only have one lot in stock of ab16496, which is GR91281-1. We are not planning on making a new lot until all10 vials of this lotin stockare sold.

We have not heard any complaints about this...

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I am sorry for the delay in getting back to you.

I can now confirm that we do not unfortunately have any antibodies directed towards the N-terminal region of PSD95 which shares high homology with the drosophila dlg-A protein.

This m...

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Thank you for getting back to me and I am sorry for the delay in getting back to you.

From the reference provided, it seems that the protein you wish to detect (dlgA) is produced from the gene dlgfrom which dlg1 isexpressed. The isoform E of ...

Read More

Thank you for contacting us and sorry for the delay in getting back to you.

I have looked intowhether theantibody you areinterested in, theanti-PSD95 (phospho T19) antibody (ab16496) would be likely to beable todetect the Drosophila protein a...

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Thank you for contacting me yesterday and this morning.

I am sorry for the delay in getting back to you but as explained over the phone I am trying to ascertain the likelihood of the PSD95 antibody being able to detect the drosophila protein....

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal cord dorsal horn lysate)
Loading amount
15 µg
Specification
Spinal cord dorsal horn lysate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

Submitted Mar 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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