Recombinant
Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-PSD95 (phospho T19) antibody [EPR2619(2)]
See all PSD95 primary antibodies -
DescriptionRabbit monoclonal [EPR2619(2)] to PSD95 (phospho T19)
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Host speciesRabbit
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Specificityab172628 only detects PSD95 phosphorylated at Threonine 19.
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSD95 aa 1-100 (phospho T19) (Cysteine residue). The exact sequence is proprietary.
Database link: P78352 -
Positive control
- Fetal brain lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR2619(2)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab172628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 80 kDa. | |
| IP | Use at an assay dependent concentration. |
Application notes
Is unsuitable for ICC or IHC-P.
Target
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FunctionInteracts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
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Tissue specificityBrain.
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Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
DomainThe PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
Post-translational
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density. -
Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
- Information by UniProt
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Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all -
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Images
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![Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)](https://www.abcam.com/ps/products/172/ab172628/Images/ab172628-196029-ab172628.jpg)
Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)All lanes : Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628) at 1/1000 dilution
Lane 1 : Fetal brain lysate, untreated
Lane 2 : Fetal brain lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 80 kDa
Datasheets and documents
References
This product has been referenced in:
- Kim DH et al. SPIN90 Modulates Long-Term Depression and Behavioral Flexibility in the Hippocampus. Front Mol Neurosci 10:295 (2017). Read more (PubMed: 28979184) »
- Beurel E et al. Ketamine-induced inhibition of glycogen synthase kinase-3 contributes to the augmentation of a-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor signaling. Bipolar Disord 18:473-480 (2016). Read more (PubMed: 27687706) »
