Name: Anti-PSD95 (phospho T19) antibody [EPR2619(2)]
Brand: RabMAb
SKU: ab172628

Product name

Anti-PSD95 (phospho T19) antibody [EPR2619(2)]
See all PSD95 primary antibodies
Description

Rabbit monoclonal [EPR2619(2)] to PSD95 (phospho T19)
Host species

Rabbit
Specificity

ab172628 only detects PSD95 phosphorylated at Threonine 19.
Tested applications

Suitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSD95 aa 1-100 (phospho T19) (Cysteine residue). The exact sequence is proprietary.
Database link: P78352
Positive control
- Fetal brain lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2619(2)
Isotype

IgG
Research areas

Alternative Versions
- Anti-PSD95 (phospho T19) antibody [EPR2619(2)] - BSA and Azide free (ab249719)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab172628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IP		Use at an assay dependent concentration.

Application notes

Is unsuitable for ICC or IHC-P.

Function

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
Tissue specificity

Brain.
Sequence similarities

Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Domain

The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
Post-translational
modifications

Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
Cellular localization

Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Target information above from: UniProt accession P78352 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
- Unigene: 27256 Mouse
- Unigene: 9765 Rat
see all
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
- Dlg4 antibody
- DLG4_HUMAN antibody
- FLJ97752 antibody
- FLJ98574 antibody
- Human post synaptic density protein 95 antibody
- Post synaptic density protein 95 antibody
- Postsynaptic density protein 95 antibody
- PSD 95 antibody
- PSD-95 antibody
- PSD95 antibody
- SAP 90 antibody
- SAP-90 antibody
- SAP90 antibody
- Synapse associated protein 90 antibody
- Synapse-associated protein 90 antibody
- Tax interaction protein 15 antibody
see all

Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)

All lanes : Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628) at 1/1000 dilution

Lane 1 : Fetal brain lysate, untreated
Lane 2 : Fetal brain lysate, treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 80 kDa

Western blot protocols
Immunoprecipitation protocols

Datasheet

This product has been referenced in:

Kim DH et al. SPIN90 Modulates Long-Term Depression and Behavioral Flexibility in the Hippocampus. Front Mol Neurosci 10:295 (2017). Read more (PubMed: 28979184) »
Beurel E et al. Ketamine-induced inhibition of glycogen synthase kinase-3 contributes to the augmentation of a-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor signaling. Bipolar Disord 18:473-480 (2016). Read more (PubMed: 27687706) »

See all 2 Publications for this product

Publishing research using ab172628? Please let us know so that we can cite the reference in this datasheet.

Location selector

Your location is currently set to:

Custom solutions & partnerships

Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As