Recombinant
RabMAb

Recombinant Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)

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Overview

  • Product name

    Anti-PSD95 (phospho T19) antibody [EPR2619(2)]
    See all PSD95 primary antibodies

  • Description

    Rabbit monoclonal [EPR2619(2)] to PSD95 (phospho T19)

  • Host species

    Rabbit

  • Specificity

    ab172628 only detects PSD95 phosphorylated at Threonine 19.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSD95 aa 1-100 (phospho T19) (Cysteine residue). The exact sequence is proprietary.
    Database link: P78352

  • Positive control

    • Fetal brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab172628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

    • Tissue specificity

      Brain.

    • Sequence similarities

      Belongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 3 PDZ (DHR) domains.
      Contains 1 SH3 domain.

    • Domain

      The PDZ domain 3 mediates interaction with ADR1B.
      The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

    • Post-translational
      modifications

      Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

    • Cellular localization

      Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Discs large homolog 4 antibody
      • Disks large homolog 4 antibody
      • DLG 4 antibody
      • Dlg4 antibody
      • DLG4_HUMAN antibody
      • FLJ97752 antibody
      • FLJ98574 antibody
      • Human post synaptic density protein 95 antibody
      • Post synaptic density protein 95 antibody
      • Postsynaptic density protein 95 antibody
      • PSD 95 antibody
      • PSD-95 antibody
      • PSD95 antibody
      • SAP 90 antibody
      • SAP-90 antibody
      • SAP90 antibody
      • Synapse associated protein 90 antibody
      • Synapse-associated protein 90 antibody
      • Tax interaction protein 15 antibody
      see all

    Images

    • Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)
      Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)
      All lanes : Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628) at 1/1000 dilution

      Lane 1 : Fetal brain lysate, untreated
      Lane 2 : Fetal brain lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 80 kDa

    References

    This product has been referenced in:

    • Kim DH  et al. SPIN90 Modulates Long-Term Depression and Behavioral Flexibility in the Hippocampus. Front Mol Neurosci 10:295 (2017). Read more (PubMed: 28979184) »
    • Beurel E  et al. Ketamine-induced inhibition of glycogen synthase kinase-3 contributes to the augmentation of a-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor signaling. Bipolar Disord 18:473-480 (2016). Read more (PubMed: 27687706) »
    See all 2 Publications for this product

    Publishing research using ab172628? Please let us know so that we can cite the reference in this datasheet.

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