Recombinant Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)
![Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)](https://www.abcam.com/ps/products/172/ab172628/Images/ab172628-196029-anti-psd95-phospho-t19-antibody-epr26192-western-blot.jpg "Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2619(2)] to PSD95 (phospho T19)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PSD95 (phospho T19) antibody [EPR2619(2)]
See all PSD95 primary antibodies -
Description
Rabbit monoclonal [EPR2619(2)] to PSD95 (phospho T19) -
Host species
Rabbit -
Specificity
ab172628 only detects PSD95 phosphorylated at Threonine 19. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSD95 aa 1-100 (phospho T19) (Cysteine residue). The exact sequence is proprietary.
Database link: P78352 -
Positive control
- Fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2619(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 80 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. |
Application notes
Is unsuitable for ICC or IHC-P.
Target
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Function
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Domain
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
Post-translational
modificationsPalmitoylation of isoform 1 is required for targeting to postsynaptic density. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
- Unigene: 463928 Human
see all -
Alternative names
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
Images
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Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)All lanes : Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628) at 1/1000 dilution
Lane 1 : Fetal brain lysate, untreated
Lane 2 : Fetal brain lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 80 kDa -
![Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)](https://www.abcam.com/ps/products/172/ab172628/Images/ab172628-2-benefits-of-recombinant-antibodies.png)
Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (ab172628)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab172628 has been referenced in 3 publications.
- Wang M et al. Decrease of GSK-3ß Activity in the Anterior Cingulate Cortex of Shank3b-/- Mice Contributes to Synaptic and Social Deficiency. Front Cell Neurosci 13:447 (2019). PubMed: 31749684
- Kim DH et al. SPIN90 Modulates Long-Term Depression and Behavioral Flexibility in the Hippocampus. Front Mol Neurosci 10:295 (2017). PubMed: 28979184
- Beurel E et al. Ketamine-induced inhibition of glycogen synthase kinase-3 contributes to the augmentation of a-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor signaling. Bipolar Disord 18:473-480 (2016). PubMed: 27687706