Overview

  • Product name

    Anti-Pseudomonas aeruginosa antibody
    See all Pseudomonas aeruginosa primary antibodies
  • Description

    Duck polyclonal to Pseudomonas aeruginosa
  • Host species

    Duck
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with Pseudomonas aeruginosa
  • Immunogen

    Pseudomonas aeruginosa whole cell

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.02% Sodium azide
    Constituents: 0.0268% PBS, 0.812% Sodium chloride
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    This antibody was purified from egg yolk. As determined by SDS-PAGE, IgY(deltaFc) constituted ~80% of total protein, and the total immunoglobulin IgY+IgY(deltaFc) constituted ~90% of total protein.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA titer: 1/5000.
    ELISA titer is defined as the dilution of antibody (1mg/ml) sufficient to give a change in absorbance of 1.0 at 450nm.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacteria are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients. P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C. P. aeruginosa is capable of growth in diesel and jet fuel, where it is known as hydrocarbon utilizing microorganisms (or "HUM bugs"), causing microbial corrosion. It creates dark gellish mats sometimes improperly called "algae".
    • Alternative names

      • P aeruginosa antibody
      • P. aeruginosa antibody
      • Pseudomonas aeruginosa antibody

    References

    This product has been referenced in:

    • Chung CY  et al. Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform. Sci Rep 7:46336 (2017). Read more (PubMed: 28402317) »
    • Chung CY  et al. Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry. PLoS One 11:e0148864 (2016). Read more (PubMed: 26863001) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply.

    I have requested further information regarding Pseudomonas aeruginosa antibody ab67905.

    I can confirm that this is known to detect 80-90% of the protein in Pseudomonas aeruginosa.

    In regard to the detection limit, the titer of the antibody has been defined by ELISA using an antigen coated well (Pseudomonas aeruginosa), so I am sorry we regrettably do not have data on the detection limit in this case.

    I can confirm that ab31138 rabbit anti Duck IgY antibody would be a suitable secondary antibody for detection of ab67805 and has been tested in ELISA.

    I am sorry we do not have all the information you require on this occasion. If you have any further questions, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for your enquiry,

    I can confirm that Pseudomonas aeruginosa antibody (ab67905) would be suitable for detection of this organism in indirect ELISA testing. I would suggest one antibody should be sufficient for detection.

    However, it should be noted that all antibodies will have a 'detection limit' and so very low levels of Pseudomonas contamination may not always be detectable.

    This antibody has not yet been tested in WB, which is why this is not listed on the datasheet. All tested applications covered by the 6 month guarantee are listed on the datasheets, and these are updated as soon as we have any new information. It does not mean it will not work, it just has not been tried yet and we cannot guarantee how well it will work. If any applications are tested unsuccessfully, this will also be listed on the individual datasheet.

    I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

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