Overview

  • Product name

  • Description

    Rabbit polyclonal to Pseudomonas
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with Pseudomonas in bacterial samples. It was raised against and validated with strain Boston 41501.
  • Tested applications

    Suitable for: ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue/ cell preparation - whole cells of Pseudomonas aeruginosa.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/800.
IHC-Fr Use at an assay dependent concentration. PubMed: 25375398

Target

  • Relevance

    Pseudomonas are Gram-negative rod bacteria commonly found in soil, ground water, plants and animals. The most common form is Pseudomonas aeruginosa which is an opportunistic pathogen of both humans and plants.

References

This product has been referenced in:

  • Walker JN  et al. The Detection of Bacteria and Matrix Proteins on Clinically Benign and Pathologic Implants. Plast Reconstr Surg Glob Open 7:e2037 (2019). Read more (PubMed: 30881821) »
  • Fernández-Blanco JA  et al. Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs. JCI Insight 3:N/A (2018). Read more (PubMed: 30185674) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Methanol

Abcam user community

Verified customer

Submitted Dec 12 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Pseudomonas aeruginosa Cell (bacteria)
Specification
bacteria
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2014

Question
Answer

Thank you for contacting us.

This antibody has not been specifically tested against Pseudomonas strains LG and GR. However, the immunogen that was used to generate this antibody was Pseudomonas aeruginosa strain ATCC #27853. Please keep in mind that the antibody has not been cross-adsorbed and it may react with other related species.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

We do not have antibody specific to Legionella species which can detect all the legionella organnisms however the antibody ab20943, is not a absorbed product so it may cross-react with related microorganisms.

I hope this information will be helpful. Should you have any other question please do not hesitate to hesitate to contact us.

Read More

Answer

Thank you for contacting us.

The following antibodies matches customer request. Please find attached the links;

https://www.abcam.com/legionella-pneumophila-antibody-ab20943.html

https://www.abcam.com/legionella-pneumophila-antibody-ab65287.html

https://www.abcam.com/pseudomonas-antibody-ab68538.html

We do not have anti Aspergillus antibodies as per customer request.

Please suggest customer to check the datasheets.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (porcine plasma-based in vitro grown biofilm)
Specification
porcine plasma-based in vitro grown biofilm
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Jan Kucera

Verified customer

Submitted Nov 15 2012

Answer

Thank you for your enquiry.

This is to confirm that the status of this Abreview has been changed from edited to submitted. It will be processed shortly by a member of our Scientific Team.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thank you for your response.

This is to confirm that the two other Abreviews have been published and the Discount codes are active. As soon as the third Abreview is submitted, its Discount code will be activated as well.

Good luck with the experiments and I am looking forward to receiving the Abreview.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry.

I have changed the status from edited to submitted of these two Abreviews (ab19980 and ab20556). They have come through to our internal system without any problem so they will be processed very shortly by one of the members of our Scientific Team.

However, it seems that the other Abreview ab68538 does not have the “Rating” information and the system recognizes it as a potential problem. I have resent this to you and ask you please look through all the information and resubmit it.

Thank you for your co-operation and I look forward to receiving your Abreviews.

Read More

Answer

DISCOUNT CODES:

ab19980 - 100%ABR-E76ZR
ab20556 - 100%ABR-E76ZL
ab68538 - 100%ABR-E7657




Expiration date: 12/11/2012

I am very pleased to hear you would like to accept our offer and testab19980, ab20556 and ab68538in IHC-P.

This code will give you: 1 per discount code free PRIMARY ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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