Key features and details
- Goat polyclonal to Pseudomonas fluorescens
- Suitable for: WB, ELISA
- Isotype: IgG
Product nameAnti-Pseudomonas fluorescens antibody
DescriptionGoat polyclonal to Pseudomonas fluorescens
Specificityab25812 recognises the majority of P. fluorescens proteins as seen by Silver Stain.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with Pseudomonas fluorescens. Not yet tested in other species.
Tissue/ cell preparation: lysates of Pseudomonas fluorescens.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Constituents: PBS, HEPES
HEPES (trace), Citric acid (trace). pH 7This antibody is provided in this buffer so the customer can perform additional modifications such as enzyme conjugation or biotinylation labeling without further processing or buffer exchange since the formulation contains no amines or other factors that might intefere in common conjugation chemistries.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was put through a Protein G IgG purification step prior to the antigen affinity purifcation step.
Our Abpromise guarantee covers the use of ab25812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Note: ab25812 is useful as either capture and/or detection antibody for direct detection of antigen.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePseudomonas fluorescens encompasses a diverse group of bacteria that is commonly found in a wide range of terrestrial and aquatic habitats. A strain that was naturally non-ice nucleating was originally isolated and registered in the USA for suppression of frost damage to fruit crops. Other strains with anti fungal or anti bacterial activity have been isolated and commercialized in the USA and India. Pseudomonas fluorescens is produced by fermentation. Its mode of action is as follows: bacteria and fungal propagules on the leaves of crops often serve as nucleation sites for ice formation and ice crystals often form when they are present and the temperature falls below freezing, with resulting damage to the leaf. If these are replaced on plant leaves with competitive antagonists (such as Pseudomonas fluorescens) that lack the ice nucleating protein, frost is prevented, even at temperatures as low as -5 °C. Other strains of Pseudomonos fluorescens are antagonistic to foliar or rhizosphere bacteria and fungi through the production of siderophores and antibiotics.
ab25812 has been referenced in 1 publication.
- Arnold CP et al. Pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of TAK1/MKK/p38. Elife 5:N/A (2016). IHC-P . PubMed: 27441386