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Microbiology Organism Virus DNA Virus double stranded DNA Virus Pseudorabies
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Anti-Pseudorabies Virus antibody (ab3534)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

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Immunohistochemistry (Frozen sections) - Anti-Pseudorabies Virus antibody (ab3534)

    Key features and details

    • Rabbit polyclonal to Pseudorabies Virus
    • Suitable for: WB, IHC-Fr, ICC
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Pseudorabies Virus antibody
    • Description

      Rabbit polyclonal to Pseudorabies Virus
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WB, IHC-Fr, ICCmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Tissue, cells or virus corresponding to Pseudorabies Virus.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Pseudorabies

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3534 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.2 - 1 µg/ml.
    IHC-Fr
    Use a concentration of 1 µg/ml.
    ICC
    Use at an assay dependent concentration.
    Notes
    WB
    Use a concentration of 0.2 - 1 µg/ml.
    IHC-Fr
    Use a concentration of 1 µg/ml.
    ICC
    Use at an assay dependent concentration.

    Target

    • Relevance

      Pseudorabies virus (PRV) is a member of the neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species. Humans and apes are refractory to PRV infection. PRV infects the epithelium of the host where it then spreads to axons of the peripheral nervous system (PNS). The virus opportunistically avails itself of the neuronal retrograde transport pathway which can facilitate transfer to the brain. This unique aspect of PRV allows it to be used as a powerful research tool to study neural connectivity and trace neuronal circuitry in the peripheral (PNS) and central nervous system (CNS).
    • Database links

      • SwissProt: P11871 Other species
      • SwissProt: P50643 Other species
      • Alternative names

        • Alphaherpesvirus pseudorabies virus antibody
        • PRV antibody
        • Suid herpesvirus 1 antibody
        • Suid herpesvirus type 1 antibody

      Images

      • Immunohistochemistry (Frozen sections) - Anti-Pseudorabies Virus antibody (ab3534)
        Immunohistochemistry (Frozen sections) - Anti-Pseudorabies Virus antibody (ab3534)
         Immunohistochemical staining of PRV in rat hypothalamus from animals infected by injection of PRV into the vitreous body of the eye with ab3534.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab3534? Please let us know so that we can cite the reference in this datasheet.

      ab3534 has been referenced in 4 publications.

      • Liu C  et al. Genetic characterization and mutation analysis of Qihe547 Aujeszky's disease virus in China. BMC Vet Res 14:218 (2018). PubMed: 29980205
      • Matthews DW  et al. Feedback in the brainstem: an excitatory disynaptic pathway for control of whisking. J Comp Neurol 523:921-42 (2015). PubMed: 25503925
      • Deshpande AJ  et al. AF10 regulates progressive H3K79 methylation and HOX gene expression in diverse AML subtypes. Cancer Cell 26:896-908 (2014). PubMed: 25464900
      • Asante CO  et al. Cortical control of adaptive locomotion in wild-type mice and mutant mice lacking the ephrin-Eph effector protein alpha2-chimaerin. J Neurophysiol 104:3189-202 (2010). IHC-FoFr . PubMed: 20881205

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry - Free Floating abreview for Anti-Pseudorabies Virus antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry free floating
      Sample
      Mouse Tissue sections (Brain and spinal cord)
      Specification
      Brain and spinal cord
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 04 2009

      Question

      Do you know if your Ab3534 will work in formalin fixed paraffin embedded tissue?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 08 2004

      Answer

      Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-formalin fixed paraffin embedded tissue. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

      Read More

      Abcam Scientific Support

      Answered on Mar 08 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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