Recombinant
RabMAb

Anti-PSF1 antibody [EPR13359] (ab181112)

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Overview

  • Product name
    Anti-PSF1 antibody [EPR13359]
    See all PSF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13359] to PSF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PSF1 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14691

  • Positive control
    • Jurkat, HeLa and HepG2 cell lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF 1/250 - 1/500.
IP 1/50.

Target

  • Function
    The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.
  • Sequence similarities
    Belongs to the GINS1/PSF1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • DNA replication complex GINS protein PSF1 antibody
      • GINS complex subunit 1 (Psf1 homolog) antibody
      • GINS complex subunit 1 antibody
      • GINS1 antibody
      • partner of sld five-1 antibody
      • partner of sld5 antibody
      • PSF1 homolog antibody
      • PSF1_HUMAN antibody
      • RP4-691N24.2 antibody
      see all

    Images

    • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
      Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
      All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
      Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    • Immunoprecipitation - Anti-PSF1 antibody [EPR13359] (ab181112)
      Immunoprecipitation - Anti-PSF1 antibody [EPR13359] (ab181112)

      Western blot analysis of PSF1 in HepG2 cell lysate immunoprecipitated with ab181112 at 1/50 dilution (Lane 1). Lane 2: Negative control.

      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    References

    ab181112 has not yet been referenced specifically in any publications.

    Publishing research using ab181112? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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