RecombinantRabMAb

Recombinant Anti-PSF1 antibody [EPR13359] (ab181112)

Submit a review Submit a question References (3)
Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Flow Cytometry - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
  • Anti-PSF1 antibody [EPR13359] (ab181112)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13359] to PSF1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PSF1 antibody [EPR13359]
    See all PSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13359] to PSF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human PSF1 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14691

  • Positive control

    • Flow Cyt: HepG2 cells ICC/IF: HeLa and HepG2 cells WB: Jurkat, HeLa and HepG2 whole cell lysate (ab7900); human fetal heart tissue lysate, mouse and rat heart tissue lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab181112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF 1/100.

For unpurified format use at 1/250 - 1/500 dilution.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.

    • Sequence similarities

      Belongs to the GINS1/PSF1 family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • DNA replication complex GINS protein PSF1 antibody
        • GINS complex subunit 1 (Psf1 homolog) antibody
        • GINS complex subunit 1 antibody
        • GINS1 antibody
        • partner of sld five-1 antibody
        • partner of sld5 antibody
        • PSF1 homolog antibody
        • PSF1_HUMAN antibody
        • RP4-691N24.2 antibody
        see all

      Images

      • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
        Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
        All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/10000 dilution (Purified)

        Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
        Lane 2 : Human fetal heart lysates
        Lane 3 : Mouse heart lysates
        Lane 4 : Rat heart lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 23 kDa
        Observed band size: 23 kDa

      • Flow Cytometry - Anti-PSF1 antibody [EPR13359] (ab181112)
        Flow Cytometry - Anti-PSF1 antibody [EPR13359] (ab181112)

        Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
        Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)

        Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/100 dilution (7.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
        Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
        All lanes : Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution (unpurified)

        Lane 1 : Jurkat cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

        Predicted band size: 23 kDa
        Observed band size: 23 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
        Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 (unpurified) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

      • Anti-PSF1 antibody [EPR13359] (ab181112)
        Anti-PSF1 antibody [EPR13359] (ab181112)

      References (3)

      Publishing research using ab181112? Please let us know so that we can cite the reference in this datasheet.

      ab181112 has been referenced in 3 publications.

      • Coulombe P  et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun 10:2426 (2019). PubMed: 31160578
      • Huang J  et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
      • Li X  et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab181112.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com