Key features and details
- Rabbit polyclonal to PSF3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSF3 antibody
See all PSF3 primary antibodies
DescriptionRabbit polyclonal to PSF3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cat, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab177515 was affinity purified using an epitope specific to PSF3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab177515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 24 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.
Sequence similaritiesBelongs to the GINS3/PSF3 family.
- Information by UniProt
- DNA replication complex GINS protein PSF3 antibody
- GINS complex subunit 3 antibody
- Gins3 antibody
Detection of PSF3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab177515 at 6 µg/mg lysate for IP and at 0.1 µg/ml for subsequent western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-PSF3 antibody (ab177515) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 3 minutes
ab177515 has been referenced in 1 publication.
- Moiseeva T et al. ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1. Nat Commun 8:1392 (2017). PubMed: 29123096