Anti-PSG1 antibody (ab233130)
Key features and details
- Rabbit polyclonal to PSG1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSG1 antibody
See all PSG1 primary antibodies -
Description
Rabbit polyclonal to PSG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human PSG1 aa 200 to the C-terminus. N-terminal tags. Expressed in E.coli.
Database link: P11464 -
Positive control
- IHC-P: Human liver, breast cancer and colorectal cancer tissue. WB: Recombinant human PSG1 protein; HeLa cell lysate; Mouse kidney and breast cancer lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
PSBG are produced in high quantity during pregnancy. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5669 Human
- Omim: 176390 Human
- SwissProt: P11464 Human
- Unigene: 709192 Human
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Alternative names
- B1G1 antibody
- Carcinoembryonic antigen SG8 antibody
- CD66 antigen-like family member F antibody
see all
Images
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All lanes : Anti-PSG1 antibody (ab233130) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse breast cancer tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for PSG1 using ab233130 at 10 µg/mL in immunohistochemical analysis.
Secondary antibody used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PSG1 using ab233130 at 10 µg/mL in immunohistochemical analysis.
Secondary antibody used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for PSG1 using ab233130 at 10 µg/mL in immunohistochemical analysis.
Secondary antibody used HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Anti-PSG1 antibody (ab233130) at 3 µg/ml + Recombinant human PSG1 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233130 has not yet been referenced specifically in any publications.