Product nameAnti-PSGL-1 antibody [EPR1967Y]
See all PSGL-1 primary antibodies
DescriptionRabbit monoclonal [EPR1967Y] to PSGL-1
Tested applicationsSuitable for: WB, IP, ICC, IHC-Gmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human PSGL-1 aa 1-100.
- Jurkat whole cell lysate (ab7899)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 43 kDa).|
|ICC||1/100 - 1/250.|
|IHC-G||Use at an assay dependent concentration. PubMed: 23635004|
FunctionA SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
(Microbial infection) Acts as a receptor for enterovirus 71.
Tissue specificityExpressed on neutrophils, monocytes and most lymphocytes.
modificationsDisplays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
- Information by UniProt
- CD 162 antibody
- CD162 antibody
- CD162 antigen antibody
ab681432(Red) staining PSGL-1 in Human bronchial tissue sections by Immunohistochemistry (Glycolmethacrylate sections). Tissue was embedded in Glycolmethacrylate and floated onto 0.2% ammonia solution prior to adherence to slides.Samples were incubated with primary antibody (1/200) for 1 hour. Samples were also stained with a neutrophil elastase antibody( Brown). Sections were counterstained with haematoxylin.
Key: E + x200 magnification and F = x500 magnification. * = Double stained cells.
All lanes : Anti-PSGL-1 antibody [EPR1967Y] (ab68143) at 1/500 dilution
Lane 1 : Jurkat cell lysate at 10 µg
Lane 2 : Recombinant PSGL-1 protein at 5ng
All lanes : HRP conjugated Goat anti-rabit at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
ab68143 has been referenced in 2 publications.
- Liu ZJ et al. SDF-1a-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia. Sci Rep 6:34416 (2016). PubMed: 27713493
- Blidberg K et al. Adhesion molecules in subjects with COPD and healthy non-smokers: a cross sectional parallel group study. Respir Res 14:47 (2013). IHC-G ; Human . PubMed: 23635004