Anti-PSGL-1 antibody [HECA-452] (ab110096)
Key features and details
- Rat monoclonal [HECA-452] to PSGL-1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgM
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PSGL-1 antibody [HECA-452]
See all PSGL-1 primary antibodies -
Description
Rat monoclonal [HECA-452] to PSGL-1 -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Human PSGL-1. Stromal preparation of human tonsils.
-
Positive control
- Human spleen or tonsil tissue. Human peripheral blood lymphocytes.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
HECA-452 -
Isotype
IgM -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml.
|
Notes |
---|
IHC-P
Use a concentration of 10 µg/ml. |
Target
-
Function
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
(Microbial infection) Acts as a receptor for enterovirus 71. -
Tissue specificity
Expressed on neutrophils, monocytes and most lymphocytes. -
Post-translational
modificationsDisplays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6404 Human
- Entrez Gene: 20345 Mouse
- Omim: 600738 Human
- SwissProt: Q14242 Human
- SwissProt: Q62170 Mouse
- Unigene: 591014 Human
- Unigene: 332590 Mouse
-
Alternative names
- CD 162 antibody
- CD162 antibody
- CD162 antigen antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab110096 has been referenced in 2 publications.
- Arnold S et al. Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues. Methods Protoc 5:N/A (2022). PubMed: 36136817
- Almaraz RT et al. Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Mol Cell Proteomics 11:M112.017558 (2012). PubMed: 22457533