Anti-PSGL-1 antibody [HECA-452] (ab110096)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)

Key features and details

  • Rat monoclonal [HECA-452] to PSGL-1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-PSGL-1 antibody [HECA-452]
    See all PSGL-1 primary antibodies

  • Description

    Rat monoclonal [HECA-452] to PSGL-1

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Other Immunogen Type corresponding to Human PSGL-1. Stromal preparation of human tonsils

  • Positive control

    • Human spleen or tonsil tissue. Human peripheral blood lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab110096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.

  • Tissue specificity

    Expressed on neutrophils, monocytes and most lymphocytes.

  • Post-translational
    modifications

    Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 162 antibody
      • CD162 antibody
      • CD162 antigen antibody
      • CLA antibody
      • Cutaneous lymphocyte associated associated antigen antibody
      • P selectin glycoprotein ligand 1 antibody
      • P selectin glycoprotein ligand 1 precursor antibody
      • P-selectin glycoprotein ligand 1 antibody
      • PSGL 1 antibody
      • PSGL-1 antibody
      • PSGL1 antibody
      • Selectin P ligand antibody
      • SELPL_HUMAN antibody
      • SELPLG antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)

      ab110096 at 10µg/ml staining PSGL-1 in Formalin/PFA-fixed paraffin-embedded Human spleen tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [HECA-452] (ab110096)

      ab110096 at 10µg/ml staining PSGL-1 in Formalin/PFA-fixed paraffin-embedded Human tonsil tissue.

    References (1)

    Publishing research using ab110096? Please let us know so that we can cite the reference in this datasheet.

    ab110096 has been referenced in 1 publication.

    • Almaraz RT  et al. Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Mol Cell Proteomics 11:M112.017558 (2012). PubMed: 22457533

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD162 antibody [HECA-452]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (LPS-inflamed lung)
    Permeabilization
    No
    Specification
    LPS-inflamed lung
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 07 2016

    Question

    I would like to test this antibody in human cells in immunocytochemistry (ICC/IF), an untested application.

    Read More
    Answer

    I am very pleased to hear you would like to accept our offer and test ab110096 in ICC/IF. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com