• Product name
  • Description
    Rabbit polyclonal to PSIP1
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: TEQQNKDEGK KPEVKKVEKK RETSMDSRLQ RIHAEIKNSL KIDNLDVNRC, corresponding to internal sequence amino acids 324-373 of Human PSIP1

  • Positive control
    • Jurkat cell lysate



Our Abpromise guarantee covers the use of ab49281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.


  • Function
    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
  • Tissue specificity
    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
  • Involvement in disease
    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities
    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • Domain
    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLL associated antigen KW 7 antibody
    • CLL-associated antigen KW-7 antibody
    • Dense fine speckles 70 kDa protein antibody
    • DFS 70 antibody
    • DFS70 antibody
    • LEDGF antibody
    • Lens epithelium derived growth factor antibody
    • Lens epithelium-derived growth factor antibody
    • MGC74712 antibody
    • p52 antibody
    • p75 antibody
    • PAIP antibody
    • PC4 and SFRS1 interacting protein 1 antibody
    • PC4 and SFRS1 interacting protein 2 antibody
    • PC4 and SFRS1 interacting protein antibody
    • PC4 and SFRS1-interacting protein antibody
    • PSIP 1 antibody
    • PSIP 2 antibody
    • Psip1 antibody
    • PSIP1_HUMAN antibody
    • PSIP2 antibody
    • Transcriptional coactivator p75/p52 antibody
    see all


  • Western blot analysis of Jurkat cell lysate labeling PSIP1 with ab49281 at 1µg/ml.
  • Western blot analysis of Jurkat cell lysate labeling PSIP1 with ab49281 at 1µg/ml.
  • Anti-PSIP1 antibody (ab49281) at 5 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa
    Observed band size: 68 kDa (why is the actual band size different from the predicted?)

    Gel concentration: 12%


This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Thank you very much for your interest in ab49281. To our knowledge, ab49281 has not been tested using a Biacore. It is not possible to submit Abreviews for this application but we would be interested in your experience with IP. Therefore, I can...

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