Product nameAnti-PSIP1 antibody [EPR11890]
See all PSIP1 primary antibodies
DescriptionRabbit monoclonal [EPR11890] to PSIP1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human PSIP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O75475
- Jurkat, HeLa and K562 cell lysates. Human brain and hepatocellular carcinoma tissues. K562 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionTranscriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
Tissue specificityWidely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
Involvement in diseaseNote=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
Sequence similaritiesBelongs to the HDGF family.
Contains 1 PWWP domain.
DomainResidues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Remains chromatin-associated throughout the cell cycle.
- Information by UniProt
- CLL associated antigen KW 7 antibody
- CLL-associated antigen KW-7 antibody
- Dense fine speckles 70 kDa protein antibody
All lanes : Anti-PSIP1 antibody [EPR11890] (ab177159) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 60 kDa
Immunofluorescent analysis of K562 cells labeling PSIP1 with ab177159 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1 with ab177159 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1 with ab177159 at 1/50 dilution.
ab177159 has not yet been referenced specifically in any publications.