Recombinant
RabMAb

Anti-PSIP1 antibody [EPR11890] (ab177159)

Overview

  • Product name
    Anti-PSIP1 antibody [EPR11890]
    See all PSIP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11890] to PSIP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PSIP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75475

  • Positive control
    • Jurkat, HeLa and K562 cell lysates. Human brain and hepatocellular carcinoma tissues. K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
    • Tissue specificity
      Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
    • Involvement in disease
      Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
    • Sequence similarities
      Belongs to the HDGF family.
      Contains 1 PWWP domain.
    • Domain
      Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Remains chromatin-associated throughout the cell cycle.
    • Information by UniProt
    • Database links
    • Alternative names
      • CLL associated antigen KW 7 antibody
      • CLL-associated antigen KW-7 antibody
      • Dense fine speckles 70 kDa protein antibody
      • DFS 70 antibody
      • DFS70 antibody
      • LEDGF antibody
      • Lens epithelium derived growth factor antibody
      • Lens epithelium-derived growth factor antibody
      • MGC74712 antibody
      • p52 antibody
      • p75 antibody
      • PAIP antibody
      • PC4 and SFRS1 interacting protein 1 antibody
      • PC4 and SFRS1 interacting protein 2 antibody
      • PC4 and SFRS1 interacting protein antibody
      • PC4 and SFRS1-interacting protein antibody
      • PSIP 1 antibody
      • PSIP 2 antibody
      • Psip1 antibody
      • PSIP1_HUMAN antibody
      • PSIP2 antibody
      • Transcriptional coactivator p75/p52 antibody
      see all

    Images

    • All lanes : Anti-PSIP1 antibody [EPR11890] (ab177159) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa

    • Immunofluorescent analysis of K562 cells labeling PSIP1 with ab177159 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1 with ab177159 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1 with ab177159 at 1/50 dilution.

    References

    ab177159 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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