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    psip1ledgf-antibody-ab244372.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Anti-PSIP1/LEDGF antibody (ab244372)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody (ab244372)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody (ab244372)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody (ab244372)

Key features and details

  • Rabbit polyclonal to PSIP1/LEDGF
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant Human PSIP1/LEDGF protein (ab82128)

View more associated products

Overview

  • Product name

    Anti-PSIP1/LEDGF antibody
    See all PSIP1/LEDGF primary antibodies
  • Description

    Rabbit polyclonal to PSIP1/LEDGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Cat
  • Immunogen

    Recombinant fragment corresponding to Human PSIP1/LEDGF aa 155-238.
    Sequence:

    KQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPC PSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQ


    Database link: O75475
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: U-2 OS cells. IHC-P: Human cerebral cortex tissue.
  • General notes

     This product was previously labelled as PSIP1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant Human PSIP1/LEDGF protein (ab82128)
  • Related Products

    • Recombinant Human PSIP1/LEDGF protein (ab82126)
    • Recombinant Human PSIP1/LEDGF protein (ab82128)

Applications

Our Abpromise guarantee covers the use of ab244372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
  • Tissue specificity

    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
  • Involvement in disease

    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities

    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • Domain

    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Target information above from: UniProt accession O75475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282011 Cow
    • Entrez Gene: 11168 Human
    • Omim: 603620 Human
    • SwissProt: Q66T72 Cat
    • SwissProt: O75475 Human
    • Unigene: 726445 Human
    • Alternative names

      • CLL associated antigen KW 7 antibody
      • CLL-associated antigen KW-7 antibody
      • Dense fine speckles 70 kDa protein antibody
      • DFS 70 antibody
      • DFS70 antibody
      • LEDGF antibody
      • Lens epithelium derived growth factor antibody
      • Lens epithelium-derived growth factor antibody
      • MGC74712 antibody
      • p52 antibody
      • p75 antibody
      • PAIP antibody
      • PC4 and SFRS1 interacting protein 1 antibody
      • PC4 and SFRS1 interacting protein 2 antibody
      • PC4 and SFRS1 interacting protein antibody
      • PC4 and SFRS1-interacting protein antibody
      • PSIP 1 antibody
      • PSIP 2 antibody
      • Psip1 antibody
      • PSIP1_HUMAN antibody
      • PSIP2 antibody
      • Transcriptional coactivator p75/p52 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody (ab244372)
      Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody (ab244372)

      PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PSIP1/LEDGF (green) using ab244372 at 4 µg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody (ab244372)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody (ab244372)

      Paraffin-embedded human cerebral cortex tissue stained for PSIP1/LEDGF using ab244372 at 1/500 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody (ab244372)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody (ab244372)

      Paraffin-embedded human liver tissue stained for PSIP1/LEDGF using ab244372 at 1/500 dilution in immunohistochemical analysis.

      Low expression as expected.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab244372? Please let us know so that we can cite the reference in this datasheet.

    ab244372 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab244372.
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