Anti-PSMA antibody [107.1A4] (ab140348)

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Overview

  • Product name

    Anti-PSMA antibody [107.1A4]
    See all PSMA primary antibodies

  • Description

    Mouse monoclonal [107.1A4] to PSMA

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue/ cell preparation of Human LNCaP cells.

  • Positive control

    • LNCaP cells.

  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27th October 2017. The following lots are still unpurified and still in stock as of 27th October 2017- GR232421-16, and GR232421-17. Lot numbers other than GR232421-16, and GR232421-17 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab140348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/5 - 1/100. Use avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

    • Tissue specificity

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.

    • Domain

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.

    • Post-translational
      modifications

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.

    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell growth inhibiting protein 27 antibody
        • Cell growth-inhibiting gene 27 protein antibody
        • FGCP antibody
        • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
        • Folate hydrolase 1 antibody
        • Folate hydrolase antibody
        • Folate hydrolase prostate specific membrane antigen 1 antibody
        • FOLH 1 antibody
        • FOLH antibody
        • Folh1 antibody
        • FOLH1_HUMAN antibody
        • Folylpoly gamma glutamate carboxypeptidase antibody
        • Folylpoly-gamma-glutamate carboxypeptidase antibody
        • GCP 2 antibody
        • GCP II antibody
        • GCP2 antibody
        • GCPII antibody
        • GIG27 antibody
        • Glutamate carboxylase II antibody
        • Glutamate carboxypeptidase 2 antibody
        • Glutamate carboxypeptidase II antibody
        • Membrane glutamate carboxypeptidase antibody
        • mGCP antibody
        • N acetylated alpha linked acidic dipeptidase 1 antibody
        • N-acetylated-alpha-linked acidic dipeptidase I antibody
        • NAALAD 1 antibody
        • NAALAD1 antibody
        • NAALAdase antibody
        • NAALADase I antibody
        • Prostate specific membrane antigen antibody
        • Prostate specific membrane antigen variant F antibody
        • Prostate-specific membrane antigen antibody
        • PSM antibody
        • PSMA antibody
        • Pteroylpoly gamma glutamate carboxypeptidase antibody
        • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [107.1A4] (ab140348)
        Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [107.1A4] (ab140348)
        Immunoflusorescent analysis of LNCaP cells labelling PSMA with ab140348.

      References

      This product has been referenced in:

      • Gardet A  et al. Pristane-Accelerated Autoimmune Disease in (SWR X NZB) F1 Mice Leads to Prominent Tubulointerstitial Inflammation and Human Lupus Nephritis-Like Fibrosis. PLoS One 11:e0164423 (2016). Read more (PubMed: 27760209) »
      See 1 Publication for this product

      Publishing research using ab140348? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Question

      Sure. I was wondering about the following product:
      Inquiry: Hello, I was wondering what the assay conditions were (e.g. fixation method) to obtain the ICC/IF image. Thanks!

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      Answer

      Thank you for your enquiry regarding ab140348.

      Precooled (- 20°C) 1:1 acetone/methanol (plastics) OR 100% aceton (glass ware) can be used as fixative. Fixation time is about 5-10 minutes at - 20°C.

      I hope this will be useful for you.

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