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    psma-antibody-1071a4-ab140348.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-PSMA antibody [107.1A4] (ab140348)

  • Datasheet
Submit a review Q&A (1)References (1)

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Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [107.1A4] (ab140348)

    Key features and details

    • Mouse monoclonal [107.1A4] to PSMA
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-PSMA antibody [107.1A4]
      See all PSMA primary antibodies
    • Description

      Mouse monoclonal [107.1A4] to PSMA
    • Host species

      Mouse
    • Tested applications

      Suitable for: ICC/IFmore details
      Unsuitable for: WB
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue/ cell preparation of Human LNCaP cells.

    • Positive control

      • LNCaP cells.
    • General notes

      We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27th October 2017. The following lots are still unpurified and still in stock as of 27th October 2017- GR232421-16, and GR232421-17. Lot numbers other than GR232421-16, and GR232421-17 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      From tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      107.1A4
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Cancer
      • Tumor biomarkers
      • Other
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Mouse PSMA protein (His tag) (ab219283)

    Applications

    Our Abpromise guarantee covers the use of ab140348 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
    • Tissue specificity

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.
    • Domain

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
    • Post-translational
      modifications

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.
    • Cellular localization

      Cytoplasm and Cell membrane.
    • Target information above from: UniProt accession Q04609 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2346 Human
      • Omim: 600934 Human
      • SwissProt: Q04609 Human
      • Unigene: 654487 Human
      • Alternative names

        • Cell growth inhibiting protein 27 antibody
        • Cell growth-inhibiting gene 27 protein antibody
        • FGCP antibody
        • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
        • Folate hydrolase 1 antibody
        • Folate hydrolase antibody
        • Folate hydrolase prostate specific membrane antigen 1 antibody
        • FOLH 1 antibody
        • FOLH antibody
        • Folh1 antibody
        • FOLH1_HUMAN antibody
        • Folylpoly gamma glutamate carboxypeptidase antibody
        • Folylpoly-gamma-glutamate carboxypeptidase antibody
        • GCP 2 antibody
        • GCP II antibody
        • GCP2 antibody
        • GCPII antibody
        • GIG27 antibody
        • Glutamate carboxylase II antibody
        • Glutamate carboxypeptidase 2 antibody
        • Glutamate carboxypeptidase II antibody
        • Membrane glutamate carboxypeptidase antibody
        • mGCP antibody
        • N acetylated alpha linked acidic dipeptidase 1 antibody
        • N-acetylated-alpha-linked acidic dipeptidase I antibody
        • NAALAD 1 antibody
        • NAALAD1 antibody
        • NAALAdase antibody
        • NAALADase I antibody
        • Prostate specific membrane antigen antibody
        • Prostate specific membrane antigen variant F antibody
        • Prostate-specific membrane antigen antibody
        • PSM antibody
        • PSMA antibody
        • Pteroylpoly gamma glutamate carboxypeptidase antibody
        • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [107.1A4] (ab140348)
        Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [107.1A4] (ab140348)
        Immunoflusorescent analysis of LNCaP cells labelling PSMA with ab140348.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab140348? Please let us know so that we can cite the reference in this datasheet.

      ab140348 has been referenced in 1 publication.

      • Gardet A  et al. Pristane-Accelerated Autoimmune Disease in (SWR X NZB) F1 Mice Leads to Prominent Tubulointerstitial Inflammation and Human Lupus Nephritis-Like Fibrosis. PLoS One 11:e0164423 (2016). PubMed: 27760209

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Sure. I was wondering about the following product:
      Inquiry: Hello, I was wondering what the assay conditions were (e.g. fixation method) to obtain the ICC/IF image. Thanks!

      Read More

      Abcam community

      Verified customer

      Asked on Dec 06 2012

      Answer

      Thank you for your enquiry regarding ab140348.

      Precooled (- 20°C) 1:1 acetone/methanol (plastics) OR 100% aceton (glass ware) can be used as fixative. Fixation time is about 5-10 minutes at - 20°C.

      I hope this will be useful for you.

      Read More

      Abcam Scientific Support

      Answered on Dec 06 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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