Overview

  • Product name

    Anti-PSMA antibody [EP3253]
    See all PSMA primary antibodies
  • Description

    Rabbit monoclonal [EP3253] to PSMA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PSMA (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: LnCaP cell lysate. ICC/IF: LnCaP cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB 1/1000 - 1/5000. Predicted molecular weight: 84 kDa.
IP 1/10.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
    • Tissue specificity

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.
    • Domain

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
    • Post-translational
      modifications

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.
    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell growth inhibiting protein 27 antibody
      • Cell growth-inhibiting gene 27 protein antibody
      • FGCP antibody
      • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
      • Folate hydrolase 1 antibody
      • Folate hydrolase antibody
      • Folate hydrolase prostate specific membrane antigen 1 antibody
      • FOLH 1 antibody
      • FOLH antibody
      • Folh1 antibody
      • FOLH1_HUMAN antibody
      • Folylpoly gamma glutamate carboxypeptidase antibody
      • Folylpoly-gamma-glutamate carboxypeptidase antibody
      • GCP 2 antibody
      • GCP II antibody
      • GCP2 antibody
      • GCPII antibody
      • GIG27 antibody
      • Glutamate carboxylase II antibody
      • Glutamate carboxypeptidase 2 antibody
      • Glutamate carboxypeptidase II antibody
      • Membrane glutamate carboxypeptidase antibody
      • mGCP antibody
      • N acetylated alpha linked acidic dipeptidase 1 antibody
      • N-acetylated-alpha-linked acidic dipeptidase I antibody
      • NAALAD 1 antibody
      • NAALAD1 antibody
      • NAALAdase antibody
      • NAALADase I antibody
      • Prostate specific membrane antigen antibody
      • Prostate specific membrane antigen variant F antibody
      • Prostate-specific membrane antigen antibody
      • PSM antibody
      • PSMA antibody
      • Pteroylpoly gamma glutamate carboxypeptidase antibody
      • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
      see all

    Images

    • Anti-PSMA antibody [EP3253] (ab76104) at 1/5000 dilution + LnCaP cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 84 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    • Confocal image showing cytoplasmic and membranous staining on LNcap cell line fixed in 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Staining PSMA with Ab76104 (1/50). Detected with ab150077 (1/1000) AlexaFluor®488 Goat anti-Rabbit secondary and counterstained with Ab195889 (1/200) Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) and DAPI.

    • Ab76104 immunoprecipitating PSMA in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates. For western blotting, primary antibody used was ab76104 at 1/1000 dilution (0.15 μg/ml). HRP labeled goat anti-rabbit IgG was used as the secondary antibody at 1/1000 dilution. Capture antibody was used at a 1/30 dilution (1.5μg in 1mg lysates).

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds

    References

    ab76104 has not yet been referenced specifically in any publications.

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