Overview

  • Product name

    Anti-PSMA antibody [EPR6253]
    See all PSMA primary antibodies
  • Description

    Rabbit monoclonal [EPR6253] to PSMA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PSMA aa 1-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human prostate carcinoma tissue; LNCaP cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
    • Tissue specificity

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.
    • Domain

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
    • Post-translational
      modifications

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.
    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell growth inhibiting protein 27 antibody
      • Cell growth-inhibiting gene 27 protein antibody
      • FGCP antibody
      • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
      • Folate hydrolase 1 antibody
      • Folate hydrolase antibody
      • Folate hydrolase prostate specific membrane antigen 1 antibody
      • FOLH 1 antibody
      • FOLH antibody
      • Folh1 antibody
      • FOLH1_HUMAN antibody
      • Folylpoly gamma glutamate carboxypeptidase antibody
      • Folylpoly-gamma-glutamate carboxypeptidase antibody
      • GCP 2 antibody
      • GCP II antibody
      • GCP2 antibody
      • GCPII antibody
      • GIG27 antibody
      • Glutamate carboxylase II antibody
      • Glutamate carboxypeptidase 2 antibody
      • Glutamate carboxypeptidase II antibody
      • Membrane glutamate carboxypeptidase antibody
      • mGCP antibody
      • N acetylated alpha linked acidic dipeptidase 1 antibody
      • N-acetylated-alpha-linked acidic dipeptidase I antibody
      • NAALAD 1 antibody
      • NAALAD1 antibody
      • NAALAdase antibody
      • NAALADase I antibody
      • Prostate specific membrane antigen antibody
      • Prostate specific membrane antigen variant F antibody
      • Prostate-specific membrane antigen antibody
      • PSM antibody
      • PSMA antibody
      • Pteroylpoly gamma glutamate carboxypeptidase antibody
      • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
      see all

    Images

    • Anti-PSMA antibody [EPR6253] (ab133579) at 1/100000 dilution (Purified) + LNCaP lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L)

      Predicted band size: 84 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?



      5% NFDM/TBST dilution buffer

    • Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

    • Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

    • Primary: ab133579, 1/50 dilution
      Dilution buffer: NFDM/TBST, 5%
      Sample: LNCaP
      Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilution



      Anti-PSMA antibody [EPR6253] (ab133579) at 1/50 dilution + LNCaP with NFDM/TBST at 5 %

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Anti-PSMA antibody [EPR6253] (ab133579) at 1/50000 dilution (Unpurified) + LNCaP cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 84 kDa

    • Unpurified ab133579 showing positive staining in Normal prostate tissue.

    • Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

    • Unpurified ab133579 showing negative staining in Normal colon tissue.

    • Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

    • Unpurified ab133579 showing negative staining in Normal spleen tissue.

    References

    This product has been referenced in:

    • Schottelius M  et al. Synthesis and Preclinical Characterization of the PSMA-Targeted Hybrid Tracer PSMA-I&F for Nuclear and Fluorescence Imaging of Prostate Cancer. J Nucl Med 60:71-78 (2019). Read more (PubMed: 30237214) »
    • Wang Z  et al. Radioligand Therapy of Prostate Cancer with a Long-Lasting Prostate-Specific Membrane Antigen Targeting Agent 90Y-DOTA-EB-MCG. Bioconjug Chem 29:2309-2315 (2018). Read more (PubMed: 29865797) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry and your interest.

    I can confirm that the concentration of this particular batch is: 0.9250 mg/ml.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

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