Recombinant
RabMAb

Recombinant Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

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Overview

  • Product name

    Anti-PSMA antibody [EPR6253] - BSA and Azide free
    See all PSMA primary antibodies

  • Description

    Rabbit monoclonal [EPR6253] to PSMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PSMA aa 1-150 (N terminal).

  • Positive control

    • Human prostate carcinoma tissue; LNCaP cell lysate

  • General notes

    Ab226141 is the carrier-free version of ab133579. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab226141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

    • Tissue specificity

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.

    • Domain

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.

    • Post-translational
      modifications

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.

    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell growth inhibiting protein 27 antibody
        • Cell growth-inhibiting gene 27 protein antibody
        • FGCP antibody
        • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
        • Folate hydrolase 1 antibody
        • Folate hydrolase antibody
        • Folate hydrolase prostate specific membrane antigen 1 antibody
        • FOLH 1 antibody
        • FOLH antibody
        • Folh1 antibody
        • FOLH1_HUMAN antibody
        • Folylpoly gamma glutamate carboxypeptidase antibody
        • Folylpoly-gamma-glutamate carboxypeptidase antibody
        • GCP 2 antibody
        • GCP II antibody
        • GCP2 antibody
        • GCPII antibody
        • GIG27 antibody
        • Glutamate carboxylase II antibody
        • Glutamate carboxypeptidase 2 antibody
        • Glutamate carboxypeptidase II antibody
        • Membrane glutamate carboxypeptidase antibody
        • mGCP antibody
        • N acetylated alpha linked acidic dipeptidase 1 antibody
        • N-acetylated-alpha-linked acidic dipeptidase I antibody
        • NAALAD 1 antibody
        • NAALAD1 antibody
        • NAALAdase antibody
        • NAALADase I antibody
        • Prostate specific membrane antigen antibody
        • Prostate specific membrane antigen variant F antibody
        • Prostate-specific membrane antigen antibody
        • PSM antibody
        • PSMA antibody
        • Pteroylpoly gamma glutamate carboxypeptidase antibody
        • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
        see all

      Images

      • Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Primary: ab133579, 1/50 dilution
        Dilution buffer: NFDM/TBST, 5%
        Sample: LNCaP
        Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilution

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing positive staining in Normal prostate tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Normal colon tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Normal spleen tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        This IHC data was generated using the same anti-PSMA antibody clone, EPR6253, in a different buffer formulation (cat# ab133579).

        Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      ab226141 has not yet been referenced specifically in any publications.

      Publishing research using ab226141? Please let us know so that we can cite the reference in this datasheet.

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