RecombinantRabMAb

Recombinant Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

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Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
  • Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6253] to PSMA - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PSMA antibody [EPR6253] - BSA and Azide free
    See all PSMA primary antibodies

  • Description

    Rabbit monoclonal [EPR6253] to PSMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment aa 1-150 (N terminal).

  • Positive control

    • Human prostate carcinoma tissue; LNCaP cell lysate

  • General notes

    Ab226141 is the carrier-free version of ab133579. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab226141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
      Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

    • Tissue specificity

      Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.

    • Domain

      The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.

    • Post-translational
      modifications

      The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
      The N-terminus is blocked.

    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell growth inhibiting protein 27 antibody
        • Cell growth-inhibiting gene 27 protein antibody
        • FGCP antibody
        • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
        • Folate hydrolase 1 antibody
        • Folate hydrolase antibody
        • Folate hydrolase prostate specific membrane antigen 1 antibody
        • FOLH 1 antibody
        • FOLH antibody
        • Folh1 antibody
        • FOLH1_HUMAN antibody
        • Folylpoly gamma glutamate carboxypeptidase antibody
        • Folylpoly-gamma-glutamate carboxypeptidase antibody
        • GCP 2 antibody
        • GCP II antibody
        • GCP2 antibody
        • GCPII antibody
        • GIG27 antibody
        • Glutamate carboxylase II antibody
        • Glutamate carboxypeptidase 2 antibody
        • Glutamate carboxypeptidase II antibody
        • Membrane glutamate carboxypeptidase antibody
        • mGCP antibody
        • N acetylated alpha linked acidic dipeptidase 1 antibody
        • N-acetylated-alpha-linked acidic dipeptidase I antibody
        • NAALAD 1 antibody
        • NAALAD1 antibody
        • NAALAdase antibody
        • NAALADase I antibody
        • Prostate specific membrane antigen antibody
        • Prostate specific membrane antigen variant F antibody
        • Prostate-specific membrane antigen antibody
        • PSM antibody
        • PSMA antibody
        • Pteroylpoly gamma glutamate carboxypeptidase antibody
        • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
        see all

      Images

      • Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Primary: ab133579, 1/50 dilution
        Dilution buffer: NFDM/TBST, 5%
        Sample: LNCaP
        Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilution

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing positive staining in Normal prostate tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Normal colon tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        Unpurified ab133579 showing negative staining in Normal spleen tissue.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

        This IHC data was generated using the same anti-PSMA antibody clone, EPR6253, in a different buffer formulation (cat# ab133579).

        Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
        Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab226141? Please let us know so that we can cite the reference in this datasheet.

      ab226141 has not yet been referenced specifically in any publications.

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