Anti-PSMA antibody [GCP-05] (ab66912)

Reviews (1)Q&A (2)References (4)

Overview

  • Product name

    Anti-PSMA antibody [GCP-05]
    See all PSMA primary antibodies

  • Description

    Mouse monoclonal [GCP-05] to PSMA

  • Host species

    Mouse

  • Tested applications

    Suitable for: IP, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA

  • Epitope

    This antibody recognizes the extracellular domain of PSMA.

  • Positive control

    • LNCaP cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab66912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 - 5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
    Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

  • Tissue specificity

    Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

  • Sequence similarities

    Belongs to the peptidase M28 family. M28B subfamily.

  • Domain

    The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.

  • Post-translational
    modifications

    The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
    The N-terminus is blocked.

  • Cellular localization

    Cytoplasm and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell growth inhibiting protein 27 antibody
      • Cell growth-inhibiting gene 27 protein antibody
      • FGCP antibody
      • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
      • Folate hydrolase 1 antibody
      • Folate hydrolase antibody
      • Folate hydrolase prostate specific membrane antigen 1 antibody
      • FOLH 1 antibody
      • FOLH antibody
      • Folh1 antibody
      • FOLH1_HUMAN antibody
      • Folylpoly gamma glutamate carboxypeptidase antibody
      • Folylpoly-gamma-glutamate carboxypeptidase antibody
      • GCP 2 antibody
      • GCP II antibody
      • GCP2 antibody
      • GCPII antibody
      • GIG27 antibody
      • Glutamate carboxylase II antibody
      • Glutamate carboxypeptidase 2 antibody
      • Glutamate carboxypeptidase II antibody
      • Membrane glutamate carboxypeptidase antibody
      • mGCP antibody
      • N acetylated alpha linked acidic dipeptidase 1 antibody
      • N-acetylated-alpha-linked acidic dipeptidase I antibody
      • NAALAD 1 antibody
      • NAALAD1 antibody
      • NAALAdase antibody
      • NAALADase I antibody
      • Prostate specific membrane antigen antibody
      • Prostate specific membrane antigen variant F antibody
      • Prostate-specific membrane antigen antibody
      • PSM antibody
      • PSMA antibody
      • Pteroylpoly gamma glutamate carboxypeptidase antibody
      • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
      see all

    Images

    • Flow Cytometry - Anti-PSMA antibody [GCP-05] (ab66912)
      Flow Cytometry - Anti-PSMA antibody [GCP-05] (ab66912)
      This image shows LNCaP cell line stained by ab66911 and ab66912. Green column - antibodies at 1µg/ml; Blue column - antibodies at 5µg/ml; Yellow column - negative cell line.

    References

    This product has been referenced in:

    • Dostalova S  et al. Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin. Sci Rep 8:8867 (2018). Read more (PubMed: 29891921) »
    • Banerjee SR  et al. 111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues. Biomacromolecules 18:201-209 (2017). Read more (PubMed: 28001364) »
    See all 4 Publications for this product

    Publishing research using ab66912? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-PSMA antibody [GCP-05]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - other (prostate)
    Gel Running Conditions
    Reduced Denaturing (4- 12.5%)
    Loading amount
    40 µg
    Specification
    prostate
    Blocking step
    Odyssey as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 26 2018

    Question

    Customer kindly called to ask whether this product would be prostate cancer tissue specific.

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately ab66912 is not tissue specific. I did however search our catalog and have found 2 products which may suit your needs. When searching for prostate cancer specific PSMA antibodies I found that the isoform expressed in normal tissue is PSMA* whereas isoforms PSMA-1 and PSMA-9 were most abundant inprostate cancer tissues oris specifically expressed in prostate cancer respectively. Therefore a search for an antibody against the N-terminal region seems the best chance for finding cancer tissue specificity as PSMA* is missing aa1-57 (http://www.uniprot.org/uniprot/Q04609).

    While we do not have data to show that the following antibodies will stain only prostate cancer tissues the immunogen regions from which they were created do match the isoforms most abundant in primary prostate tumors and not with the form found in normal prostate.

    Ab76104: The immunogen is located within aa1-25. As such this product will only recognize isoform 1 and isoform 8.The product page for ab76104 is at:https://www.abcam.com/PSMA-antibody-EP3253-ab76104.html

    Ab41034: The immunogen region is from aa43- aa60 so this is likely not to recognize PSMA* but will recognizePSMA-1 and PSMA-9. The product page is located at this address:https://www.abcam.com/PSMA-antibody-ab41034.html


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Read More

    Question

    I am currently trying to identify the levels of PSMA expression in human cell and tumor lysates by western blot analysis in both reducing and native conditions and was wondering if you have a primary antibody that would be useful in both of these applications? I have been using your YPSMA antibody but have found differing expression levels for the same cells under reducing and native conditions and would like to optimize this assay as much as possible.

    Any information you can give would be greatly appreciated,

    Read More
    Answer

    Thank you for contacting us.

    We do not have anti PSMA antibody that would detect both the native as well as reduced form of protein. We however have more than 20 products that are used under reducing conditions and gave best results. Taking ion consideration the current data we have, It is hard to tell which antibody is suitable for native protein, theoretically every antibody should detect however there are certain antibodies that works well under denatured and reduced conditions rather than native.

    I am sorry we are not aware about our PSMA antibodies for their use under native conditions. This has to determined by the end user.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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