- Datasheet
- Specific References (2)
- Protocols
Overview
-
Product nameAnti-PSMA antibody [GCP-05]
See all PSMA primary antibodies -
DescriptionMouse monoclonal [GCP-05] to PSMA
-
Host speciesMouse
-
Tested applicationsSuitable for: IP, ICC, Flow Cytmore details
-
Species reactivityReacts with: Human
-
Immunogen
Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA
-
EpitopeThis antibody recognizes the extracellular domain of PSMA.
-
Positive control
- LNCaP cells.
-
General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
-
Purification notesPurity: > 95% Purified from TCS
-
ClonalityMonoclonal
-
Clone numberGCP-05
-
IsotypeIgG1
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab66912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 1 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
-
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
-
DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
-
Post-translational
modificationsThe first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
-
Alternative names
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
Images
-
![Flow Cytometry - Anti-PSMA antibody [GCP-05] (ab66912)](https://www.abcam.com/ps/products/66/ab66912/Images/ab66912-1.jpg)
Flow Cytometry - Anti-PSMA antibody [GCP-05] (ab66912)This image shows LNCaP cell line stained by ab66911 and ab66912. Green column - antibodies at 1µg/ml; Blue column - antibodies at 5µg/ml; Yellow column - negative cell line.
Protocols
References
This product has been referenced in:
- Dostalova S et al. Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin. Sci Rep 8:8867 (2018). Read more (PubMed: 29891921) »
- Castanares MA et al. Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. Prostate 76:215-25 (2016). Read more (PubMed: 26499105) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"