Key features and details
- Mouse monoclonal [YPSMA-1] to PSMA
- Suitable for: ELISA, WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-PSMA antibody [YPSMA-1]
See all PSMA primary antibodies
DescriptionMouse monoclonal [YPSMA-1] to PSMA
Tested applicationsSuitable for: ELISA, WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Crude membrane protein preparation from pooled prostate malignant carcinoma from China.
- LNCaP cell line, recombinant PSMA.
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by June 2020 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab133579 or ab76104 as possible replacements.
This antibody detects PMSA in prostate cancer but shows little or no cross-reactivity to benign prostate hyperplasia or to normal prostatic tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.00
Concentration information loading...
Purification notesPurified from ascites.
Primary antibody notesThis antibody detects PMSA in prostate cancer but shows little or no cross-reactivity to benign prostate hyperplasia or to normal prostatic tissue.
Light chain typekappa
Our Abpromise guarantee covers the use of ab19071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionHas both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Tissue specificityHighly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
DomainThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
modificationsThe first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
- Cell growth inhibiting protein 27 antibody
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Overlay histogram showing LnCAP cells stained with ab19071 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19071, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in LnCAP cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemistry of formalin-fixed paraffin embedded prostate cancer tissue sections labeling PSMA with ab19071 at 1:1000 dilution.
ab19071 has been referenced in 15 publications.
- Shirazi HS et al. Plasma enabled devices for the selective capture and photodynamic identification of prostate cancer cells. Biointerphases 15:031002 (2020). PubMed: 32414237
- Morgenroth A et al. Targeting of prostate-specific membrane antigen for radio-ligand therapy of triple-negative breast cancer. Breast Cancer Res 21:116 (2019). PubMed: 31640747
- Endepols H et al. Peripheral ganglia in healthy rats as target structures for the evaluation of PSMA imaging agents. BMC Cancer 19:633 (2019). PubMed: 31242896
- Dowling M et al. Overexpression of prostate specific membrane antigen by canine hemangiosarcoma cells provides opportunity for the molecular detection of disease burdens within hemorrhagic body cavity effusions. PLoS One 14:e0210297 (2019). PubMed: 30601866
- Nguyen T et al. Uncovering the Role of N-Acetyl-Aspartyl-Glutamate as a Glutamate Reservoir in Cancer. Cell Rep 27:491-501.e6 (2019). PubMed: 30970252