Overview

  • Product name
    Anti-PSMA antibody [YPSMA-1]
    See all PSMA primary antibodies
  • Description
    Mouse monoclonal [YPSMA-1] to PSMA
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Crude membrane protein preparation from pooled prostate malignant carcinoma from China.

  • Positive control
    • LNCaP cell line, recombinant PSMA.
  • General notes

    This antibody detects PMSA in prostate cancer but shows little or no cross-reactivity to benign prostate hyperplasia or to normal prostatic tissue.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab19071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
    Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
  • Tissue specificity
    Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
  • Sequence similarities
    Belongs to the peptidase M28 family. M28B subfamily.
  • Domain
    The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
  • Post-translational
    modifications
    The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth inhibiting protein 27 antibody
    • Cell growth-inhibiting gene 27 protein antibody
    • FGCP antibody
    • Folate hydrolase (prostate-specific membrane antigen) 1 antibody
    • Folate hydrolase 1 antibody
    • Folate hydrolase antibody
    • Folate hydrolase prostate specific membrane antigen 1 antibody
    • FOLH 1 antibody
    • FOLH antibody
    • Folh1 antibody
    • FOLH1_HUMAN antibody
    • Folylpoly gamma glutamate carboxypeptidase antibody
    • Folylpoly-gamma-glutamate carboxypeptidase antibody
    • GCP 2 antibody
    • GCP II antibody
    • GCP2 antibody
    • GCPII antibody
    • GIG27 antibody
    • Glutamate carboxylase II antibody
    • Glutamate carboxypeptidase 2 antibody
    • Glutamate carboxypeptidase II antibody
    • Membrane glutamate carboxypeptidase antibody
    • mGCP antibody
    • N acetylated alpha linked acidic dipeptidase 1 antibody
    • N-acetylated-alpha-linked acidic dipeptidase I antibody
    • NAALAD 1 antibody
    • NAALAD1 antibody
    • NAALAdase antibody
    • NAALADase I antibody
    • Prostate specific membrane antigen antibody
    • Prostate specific membrane antigen variant F antibody
    • Prostate-specific membrane antigen antibody
    • PSM antibody
    • PSMA antibody
    • Pteroylpoly gamma glutamate carboxypeptidase antibody
    • Pteroylpoly-gamma-glutamate carboxypeptidase antibody
    see all

Images

  • Overlay histogram showing LnCAP cells stained with ab19071 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19071, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in LnCAP cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemistry of formalin-fixed paraffin embedded prostate cancer tissue sections labeling PSMA with ab19071 at 1:1000 dilution.

References

This product has been referenced in:
  • Tao R  et al. Expression, purification and identification of an immunogenic fragment in the ectodomain of prostate-specific membrane antigen. Exp Ther Med 11:747-752 (2016). Read more (PubMed: 26997988) »
  • Diao Y  et al. A specific aptamer-cell penetrating peptides complex delivered siRNA efficiently and suppressed prostate tumor growth in vivo. Cancer Biol Ther 17:498-506 (2016). Read more (PubMed: 26954374) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP C42B and WMPY1 prostate cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
LNCaP C42B and WMPY1 prostate cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Dimitra Kalamida

Verified customer

Submitted Jun 18 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution low pH
Permeabilization
Yes - 0.1% Triton X-100
Specification
Prostate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formalin

Dr. Dimitra Kalamida

Verified customer

Submitted Jun 01 2018

Answer

Thank you for your email.
Yes, that is correct. Alternatively, you can just use a secondary antibody that has already been conjugated with HRP. We carry many in our catalog. Please let me know if you need help selecting one.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

The conjugation kit ab102891 is compatible with antibody formulations that contain up to 1% BSA, even though the BSA is in 10-fold excess to the antibody (10mg/ml BSA compared to 1 mg/ml IgG for ab71720). Some of the BSA will itself will be conjugated, affecting efficient conjugation of the IgG, so an antibody without BSA would be better, such as anti-PSMA ab19071.

However, even with 1% BSA, much of the IgG in ab71720 can be conjugated to HRP using the HRP kit ab102891, and will be useful for subsequent assays. I do not know if that is the case for the other company's conjugation kit.

Read More

Answer

I think the CEA antibody formulation will be compatible with the other company's labeling kit but you should contact them and send them the buffer components. Here are the components of the ab15987 buffer:

Preservative: 15mM Sodium Azide Constituents: 0.1M Sodium chloride, pH 7.2

The concentration of IgG for our current lot of ab15987 is 2.0 mg/ml. There is no protein carrier such as BSA.

The PSMA antibody formulation (ab97080) may be more problematic, as it contains BSA, which may interfere with conjugation of the HRP to the immunoglobulin. The buffer components are:

Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4

The IgG concentration of ab97080 is 1.0 mg/ml.

Again, the other company will be able to tell you if these components are compatible with their conjugation kit. You may need to remove the BSA, in which case I recommend our purification product, ab102784.

https://www.abcam.com/index.html?datasheet=102784

We also have an HRP conjugation kit, if you are interested. The catalogue number is ab102891.

https://www.abcam.com/index.html?datasheet=102891

I hope this is helpful. If you have any questions, please contact us.

Read More

Answer

Thank you for contacting us.

We do not have anti PSMA antibody that would detect both the native as well as reduced form of protein. We however have more than 20 products that are used under reducing conditions and gave best results. Taking ion consideration the current data we have, It is hard to tell which antibody is suitable for native protein, theoretically every antibody should detect however there are certain antibodies that works well under denatured and reduced conditions rather than native.

I am sorry we are not aware about our PSMA antibodies for their use under native conditions. This has to determined by the end user.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

To your first question about using Anti-PSMA antibody ab19071 in Sandwich ELISA (sELISA):
We unfortunately have not tested if this PSMA antibody works in Sandwich ELISA and therefore cannot recommend a specific concentration range. It usually depends on the type of plates or the samples you use and other factors. In general, a concentration of 1 to 10 µg/ml in carbonate/bicarbonate buffer (pH7.4) is recommended. However, this needs to be optimised for your individual experimental setting. I have attached our protocol which might be of further use for you.

To your second question about the immunogen:
Indeed, this antibody detects the peptide sequence ESKVDPSK which is derived from amino acids 716-723 of the PSMA.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application (as in this case for sELISA), you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Dear Sir:
Thank you for your kind reply. My samples are human derived (human blood and serum or urine). I am interested in Sandwich ELISA for Carcinoembryonic Antigen and Prostate-specific membrane antigen.
I am waiting for your suggestion on primary, secondary antibodies and antigenfor the samewhich are available with you.
Regards
Sunil Bhand

On Thu, Mar 1, 2012 at 5:52 AM, <mailto:technical@abcam.com> wrote:



Dear Dr Bhand,

We have an answer to your inquiry:
Thank you for contacting us. I will be happy to make some suggestions. Can you please confirm that your samples are human-derived, and that you are interested in a sandwich ELISA, rather than direct or indirect ELISA?

For an example of a sandwich ELISA antibody pair that we recommend, two antibodies that have been tested together for detection of human CEA by sandwich ELISA are ab4451 (for capture onto the plate) and ab15987 (for detection). The rabbit polyclonal ab15987 is unconjugated, so you will need to detect it in turn with an anti-rabbit IgG antibody, such as the HRP conjugate ab97080.

For the CEA antigen, and for the PSA assay, we have several choices but I will wait for your information regarding your samples and assay requirements before making suggestions.
Help us improve our service. Rate your experience with us today. https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3557625

Your original inquiry to Abcam:
-------------------------------------------

I want to perform ELISA for Carcinoembryonic Antigen and Prostate-specific membrane antigen. Please suggest me the suitable primary as well as secondary conjugated antibodies for both. Also I want to procure the said antigens and antibodies.
Kindly send me the quotations for the above mentioned products.

-------------------------------------------


Best regards,
Thomas

Thomas Ruyle
Scientific Support Specialist
Abcam Inc.
https://www.abcam.com


Abcam Customer Services and Technical Support Team

https://www.abcam.com/technical
[CCE3557625]

--
Thanks & Regards
Deepti Parab
Office Assistant to
Dr. Sunil Bhand
Department of Chemistry
NAIP Project, BITS Pilani-K.K.Birla Goa Campus
Contact : 0832 2580340

Read More
Answer

For the CEA assay, I recommend the antibodies I mentioned in my previous e-mail, ab4451 for capture and ab15987 for detection, followed by the secondary, anti-rabbit IgG conjugated to HRP, ab97080, and appropriate substrate, for instance, TMB.
For CEA antigen, I recommend ab724:

https://www.abcam.com/index.html?datasheet=724

For the PSMA assay, we do not have a pair of antibodies that has been tested together in sandwich ELISA. However, the following two antibodies are likely to be an appropriate pair, as they are raised against different portions of the PSMA protein: ab19071 for capture, and ab71720 for detection, followed by ab97080, the rabbit IgG-specific HRP conjugate, and substrate.

However, antibody ab71720 has not been tested in ELISA, and I cannot guarantee it will work well in this application, though the western blot looks very good.

We do not have an appropriate PSMA protein in our catalogue, so I suggest searching the website www.biocompare.com.

I hope this helps. If you have any questions, please contact me. For prices, please consult the online datasheets.

Read More

Answer

Thank you for your enquiry. I can confirm that the predicted molecular weight of PSMA is around 84 kDa. However, the actual band size of the mature protein can be different from the theoretical size. According to Swiss-Prot database, there are at least 9 splice variants and the processed protein can be heavily N-Glycosylated (10 different potential positions have been described i.e. 51, 76, 121, 140, 153, 195, 336, 459, 476, 638). Depending on the complex city of the polysaccharide chain, it is possible that the actual band size can be around 120-130 kDa. You may wish to take a look at this site for further details: http://www.uniprot.org/uniprot/Q04609 I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate, mixed population)
Specification
prostate, mixed population
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

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Verified customer

Submitted Oct 14 2009

1-10 of 17 Abreviews or Q&A

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