Product nameAnti-PSMA3 antibody
See all PSMA3 primary antibodies
DescriptionRabbit polyclonal to PSMA3
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Recombinant full length protein corresponding to Human PSMA3 aa 1-255.
MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGV EKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFR SNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLY MIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYI VHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDES DDDNM
Database link: P25788
- JAR, A549 and PC12 cell extracts.
This product was previously labelled as Proteasome 20S alpha 3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 28 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC8 antibody
- Macropain subunit C8 antibody
- MGC12306 antibody
All lanes : Anti-PSMA3 antibody (ab180784) at 1/1000 dilution
Lane 1 : MCF7 cell lysate with 3% nonfat dry milk in TBST
Lane 2 : DU145 cell lysate with 3% nonfat dry milk in TBST
Lane 3 : HL-60 cell lysate with 3% nonfat dry milk in TBST
Lane 4 : PC-3 cell lysate with 3% nonfat dry milk in TBST
Lane 5 : Mouse liver lysate with 3% nonfat dry milk in TBST
Lane 6 : Mouse spleen lysate with 3% nonfat dry milk in TBST
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Immunoprecipitation analysis of 200 μg extracts of HL-60 cells, using 3 μg of ab180784. Western blot was performed from the immunoprecipitate using ab180784 at a dilition of 1:1000.
All lanes : Anti-PSMA3 antibody (ab180784) at 1/500 dilution
Lane 1 : JAR cell extracts
Lane 2 : A549 cell extracts
Lane 3 : PC12 cell extracts
Predicted band size: 28 kDa
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180784. Blue DAPI for nuclear staining.
ab180784 has not yet been referenced specifically in any publications.