Overview

  • Product name

    Anti-PSMA3 antibody [EPR5455]
    See all PSMA3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5455] to PSMA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMA3 aa 50-150. The exact sequence is proprietary.
    Database link: P25788

  • Positive control

    • A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
  • General notes

     

     

     This product was previously labelled as Proteasome 20S alpha 3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5455
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
    • Sequence similarities

      Belongs to the peptidase T1A family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HC8 antibody
      • Macropain subunit C8 antibody
      • MGC12306 antibody
      • MGC32631 antibody
      • Multicatalytic endopeptidase complex subunit C8 antibody
      • Proteasome (prosome macropain) subunit alpha type 3 antibody
      • Proteasome alpha 3 subunit antibody
      • Proteasome component C8 antibody
      • Proteasome subunit alpha type 3 antibody
      • Proteasome subunit alpha type-3 antibody
      • Proteasome subunit C8 antibody
      • PSA3_HUMAN antibody
      • PSC8 antibody
      • psmA3 antibody
      see all

    Images

    • All lanes : Anti-PSMA3 antibody [EPR5455] (ab109532) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • ab109532, at 1/100 dilution, staining PSMA3 in paraffin embedded Human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • ab109532, at 1/100 dilution, staining PSMA3 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Takahashi M  et al. USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis. iScience 9:433-450 (2018). Read more (PubMed: 30469013) »
    • Li Y  et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Sci Rep 7:40698 (2017). WB ; Chicken . Read more (PubMed: 28079188) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 14 2019

    Answer

    We do not have any data suggesting that the antibody has higher affinity for nuclear PSMA3 than for cytoplasmic PSMA3. The differences in staining intensity may be due to unequal distribution of the protein (more PSMA3 in the nucleus than in the cytoplasm), or the epitope that the antibody recognizes on PSMA3 may be obscured in the nucleus, depending on what it is complexed with, compared to what it is complexed with in the cytoplasm.

    A western blot of denatured subcellular fractions might help clarify which is the case. The antibody is raised against a peptide sequence which, after denaturation of the samples, will be available for the antibody to bind to, without interference from proteosome complexes that are unique to the nucleus or cytoplasm.

    On the other hand, the antibody may simply be cross-reacting with something other than PSMA3. It is, however, expected to be present in both the nucleus and the cytoplasm., and what apppears to be cytoplasmic background may in fact be PSMA3.

    Read More

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