Product nameAnti-PSMA3 (phospho S250) antibody
See all PSMA3 primary antibodies
DescriptionRabbit polyclonal to PSMA3 (phospho S250)
Detects endogenous levels of PSMA3 only when phosphorylated at serine 250.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human PSMA3 (phospho S250).
Database link: P25788
- Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)
This product was previously labelled as Proteasome 20S alpha 3
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab79184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 28 kDa.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC8 antibody
- Macropain subunit C8 antibody
- MGC12306 antibody
All lanes : Anti-PSMA3 (phospho S250) antibody (ab79184) at 1/500 dilution
Lane 1 : Extracts from 3T3 cells, treated
with EGF (200 ng/ml, 30 minutes)
Lane 2 : Extracts from 3T3 cells, treated
with EGF (200 ng/ml, 30 minutes) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
ab79184 has not yet been referenced specifically in any publications.