Recombinant
RabMAb

Recombinant Anti-PSMA4 antibody [EPR5831(2)] - BSA and Azide free (ab236158)

Overview

  • Product name

    Anti-PSMA4 antibody [EPR5831(2)] - BSA and Azide free
    See all PSMA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR5831(2)] to PSMA4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMA4 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P25789

  • Positive control

    • Jurkat whole cell lysate (ab7899) can be used as a positive control in WB. IHC-P: Human papillary adenocarcinoma of thyroid tissue.
  • General notes

    Ab236158 is the carrier-free version of ab191403. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236158 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5831(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similarities

    Belongs to the peptidase T1A family.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm, P-body. Colocalizes with TRIM5 in the cytoplasmic bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • HC9 antibody
    • HsT17706 antibody
    • Macropain subunit C9 antibody
    • MGC111191 antibody
    • MGC12467 antibody
    • MGC24813 antibody
    • Multicatalytic endopeptidase complex subunit C9 antibody
    • Proteasome (prosome macropain) subunit alpha type 4 antibody
    • Proteasome alpha 4 subunit antibody
    • Proteasome component C9 antibody
    • Proteasome subunit alpha type 4 antibody
    • Proteasome subunit alpha type-4 antibody
    • Proteasome subunit HC9 antibody
    • Proteasome subunit L antibody
    • PSA4_HUMAN antibody
    • PSC9 antibody
    • PSMA 4 antibody
    • psmA4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191403).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PSMA4 with ab191403 at a 1/500 dilution ( 4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PSMA4 at a 1/500 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191403).

  • Western blot analysis on immunoprecipitation from 1) Jurkat cell lysate and 2) PBS, labeling PSMA4 using ab191403 at 1/200 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191403).

  • Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191403).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab236158 has not yet been referenced specifically in any publications.

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