Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMA7/HSPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human PSMA7/HSPC aa 1-175.
    Database link: O14818

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IHC-P: DLD-1 xenograft
  • General notes

     This product was previously labelled as PSMA7

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
  • Sequence similarities

    Belongs to the peptidase T1A family.
  • Post-translational
    modifications

    Phosphorylation by ABL1 or ABL2 leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C6 antibody
    • HSPC antibody
    • MGC3755 antibody
    • OTTHUMP00000031449 antibody
    • OTTHUMP00000031450 antibody
    • OTTHUMP00000031453 antibody
    • Proteasome (prosome macropain) subunit alpha type 7 antibody
    • Proteasome alpha 7 subunit antibody
    • Proteasome subunit alpha 4 antibody
    • Proteasome subunit alpha type 7 antibody
    • Proteasome subunit alpha type-7 antibody
    • Proteasome subunit RC6 1 antibody
    • Proteasome subunit RC6-1 antibody
    • Proteasome subunit XAPC7 antibody
    • PSA7_HUMAN antibody
    • PSMA 7 antibody
    • PSMA7 antibody
    • RC6 1 antibody
    • XAPC7 antibody
    see all

Images

  • All lanes : Anti-PSMA7/HSPC antibody (ab97565) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 28 kDa



    12% SDS PAGE
  • ab97565, at a 1/100 dilution, staining PSMA7/HSPC in a paraffin-embedded DLD-1 xenograft by Immunohistochemical analysis.

References

ab97565 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - Triton
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 19 2016

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