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Immunology Cell Type Markers CD Myeloid Cells
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RecombinantRabMAb

Recombinant Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)

  • Datasheet
  • SDS
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Western blot - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
  • OI-RD Scanning - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
  • Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5836] to PSMA7/HSPC
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Recombinant Human PSMA7/HSPC protein (ab81381)

View more associated products

Overview

  • Product name

    Anti-PSMA7/HSPC antibody [EPR5836]
    See all PSMA7/HSPC primary antibodies
  • Description

    Rabbit monoclonal [EPR5836] to PSMA7/HSPC
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human PSMA7/HSPC aa 1-100. The exact sequence is proprietary.
    Database link: O14818

  • Positive control

    • A549, MCF7, 293T, and HeLa cell lysates, Human breast carcinoma tissue
  • General notes

     

     

     This product was previously labelled as PSMA7

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5836
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Alternative Versions

    • Anti-PSMA7/HSPC antibody [EPR5836] - BSA and Azide free (ab248526)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
    • HeLa membrane extract lysate (ab29547)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human PSMA7/HSPC protein (ab81381)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
    • Sequence similarities

      Belongs to the peptidase T1A family.
    • Post-translational
      modifications

      Phosphorylation by ABL1 or ABL2 leads to an inhibition of proteasomal activity and cell cycle transition blocks.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession O14818 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5688 Human
      • Entrez Gene: 26444 Mouse
      • Entrez Gene: 29674 Rat
      • Omim: 606607 Human
      • SwissProt: O14818 Human
      • SwissProt: Q9Z2U0 Mouse
      • SwissProt: P48004 Rat
      • Unigene: 233952 Human
      • Unigene: 21728 Mouse
      • Unigene: 105784 Rat
      see all
    • Alternative names

      • C6 antibody
      • HSPC antibody
      • MGC3755 antibody
      • OTTHUMP00000031449 antibody
      • OTTHUMP00000031450 antibody
      • OTTHUMP00000031453 antibody
      • Proteasome (prosome macropain) subunit alpha type 7 antibody
      • Proteasome alpha 7 subunit antibody
      • Proteasome subunit alpha 4 antibody
      • Proteasome subunit alpha type 7 antibody
      • Proteasome subunit alpha type-7 antibody
      • Proteasome subunit RC6 1 antibody
      • Proteasome subunit RC6-1 antibody
      • Proteasome subunit XAPC7 antibody
      • PSA7_HUMAN antibody
      • PSMA 7 antibody
      • PSMA7 antibody
      • RC6 1 antibody
      • XAPC7 antibody
      see all

    Images

    • Western blot - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
      Western blot - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
      All lanes : Anti-PSMA7/HSPC antibody [EPR5836] (ab133502) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)

      Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling PSMA7/HSPC using ab133502 antibody at a dilution of 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
      OI-RD Scanning - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)
      Anti-PSMA7/HSPC antibody [EPR5836] (ab133502)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab133502? Please let us know so that we can cite the reference in this datasheet.

    ab133502 has been referenced in 2 publications.

    • Ren CC  et al. Effects of shRNA-mediated silencing of PSMA7 on cell proliferation and vascular endothelial growth factor expression via the ubiquitin-proteasome pathway in cervical cancer. J Cell Physiol 234:5851-5862 (2019). PubMed: 29247526
    • Nath SR  et al. Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction. J Clin Invest 128:3630-3641 (2018). PubMed: 29809168

    Customer reviews and Q&As

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