Overview

  • Product name

    Anti-PSMA7/HSPC antibody [EPR5836]
    See all PSMA7/HSPC primary antibodies
  • Description

    Rabbit monoclonal [EPR5836] to PSMA7/HSPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMA7/HSPC aa 1-100. The exact sequence is proprietary.
    Database link: O14818

  • Positive control

    • A549, MCF7, 293T, and HeLa cell lysates, Human breast carcinoma tissue
  • General notes

     

     

     This product was previously labelled as PSMA7

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
    • Sequence similarities

      Belongs to the peptidase T1A family.
    • Post-translational
      modifications

      Phosphorylation by ABL1 or ABL2 leads to an inhibition of proteasomal activity and cell cycle transition blocks.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C6 antibody
      • HSPC antibody
      • MGC3755 antibody
      • OTTHUMP00000031449 antibody
      • OTTHUMP00000031450 antibody
      • OTTHUMP00000031453 antibody
      • Proteasome (prosome macropain) subunit alpha type 7 antibody
      • Proteasome alpha 7 subunit antibody
      • Proteasome subunit alpha 4 antibody
      • Proteasome subunit alpha type 7 antibody
      • Proteasome subunit alpha type-7 antibody
      • Proteasome subunit RC6 1 antibody
      • Proteasome subunit RC6-1 antibody
      • Proteasome subunit XAPC7 antibody
      • PSA7_HUMAN antibody
      • PSMA 7 antibody
      • PSMA7 antibody
      • RC6 1 antibody
      • XAPC7 antibody
      see all

    Images

    • All lanes : Anti-PSMA7/HSPC antibody [EPR5836] (ab133502) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa
      Observed band size: 27 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling PSMA7/HSPC using ab133502 antibody at a dilution of 1/250.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Nath SR  et al. Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction. J Clin Invest 128:3630-3641 (2018). Read more (PubMed: 29809168) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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