Overview

  • Product name

    Anti-PSMA7/HSPC antibody [EPR5837]
    See all PSMA7/HSPC primary antibodies
  • Description

    Rabbit monoclonal [EPR5837] to PSMA7/HSPC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMA7/HSPC aa 200-300. The exact sequence is proprietary.
    Database link: O14818

  • Positive control

    • WB: MCF7, HeLa, HepG2 and A549 cell lysates IHC-P: Human kidney carcinoma tissue
  • General notes

     

     

     This product was previously labelled as PSMA7

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5837
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
    • Sequence similarities

      Belongs to the peptidase T1A family.
    • Post-translational
      modifications

      Phosphorylation by ABL1 or ABL2 leads to an inhibition of proteasomal activity and cell cycle transition blocks.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C6 antibody
      • HSPC antibody
      • MGC3755 antibody
      • OTTHUMP00000031449 antibody
      • OTTHUMP00000031450 antibody
      • OTTHUMP00000031453 antibody
      • Proteasome (prosome macropain) subunit alpha type 7 antibody
      • Proteasome alpha 7 subunit antibody
      • Proteasome subunit alpha 4 antibody
      • Proteasome subunit alpha type 7 antibody
      • Proteasome subunit alpha type-7 antibody
      • Proteasome subunit RC6 1 antibody
      • Proteasome subunit RC6-1 antibody
      • Proteasome subunit XAPC7 antibody
      • PSA7_HUMAN antibody
      • PSMA 7 antibody
      • PSMA7 antibody
      • RC6 1 antibody
      • XAPC7 antibody
      see all

    Images

    • All lanes : Anti-PSMA7/HSPC antibody [EPR5837] (ab109378) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa
      Observed band size: 27 kDa
      why is the actual band size different from the predicted?

    • ab109378, at a 1/250 dilution, staining Proteasome 20S alpha 7 in paraffin embedded Human kidney carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab109378 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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