Anti-PSMB1 antibody (ab196623)
Key features and details
- Rabbit polyclonal to PSMB1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PSMB1 antibody
See all PSMB1 primary antibodies -
Description
Rabbit polyclonal to PSMB1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human PSMB1.
Database link: P20618 -
Positive control
- MCF7, HL-60, SW480, K562, PC3, mouse skeletal muscle and mouse liver cell line extracts. Human breast cancer and rat testis tissue. U20S cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+), -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 26 kDa.
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 26 kDa. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
Sequence similarities
Belongs to the peptidase T1B family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5689 Human
- Entrez Gene: 19170 Mouse
- Entrez Gene: 94198 Rat
- Omim: 602017 Human
- SwissProt: P20618 Human
- SwissProt: O09061 Mouse
- SwissProt: P18421 Rat
- Unigene: 352768 Human
see all -
Alternative names
- FLJ25321 antibody
- HC5 antibody
- KIAA1838 antibody
see all
Images
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All lanes : Anti-PSMB1 antibody (ab196623) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : HL60 cell extract
Lane 3 : SW480 cell extract
Lane 4 : HepG2 cell extract
Lane 5 : SW620 cell extract
Lane 6 : K562 cell extract
Lane 7 : PC3 cell extract
Lane 8 : Mouse skeletal muscle cell extract
Lane 9 : Mouse liver cell extract
Developed using the ECL technique.
Predicted band size: 26 kDa -
Immunofluorscence analysis of U20S cells labeling PSMB1 with ab196623 at 1/50 dilution (top panel).
Blue: DAPI for nuclear staining (bottom panel).
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Immunohistochemical analysis of formalin fixed and paraffin embedded rat testis labeling PSMB1 with ab196623 at a 1/200 dilution.
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Immunohistochemical analysis of formalin fixed and paraffin embedded human breast cancer tissue labeling PSMB1 with ab196623 at a 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196623 has been referenced in 1 publication.
- Woodle ES et al. Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells. Am J Transplant 20:399-410 (2020). PubMed: 31595669