Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMB1
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous level of total PSMB1 protein.

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human PSMB1.
    Database link: P20618

  • Positive control

    • MCF7, HL-60, SW480, K562, PC3, mouse skeletal muscle and mouse liver cell line extracts. Human breast cancer and rat testis tissue. U20S cells.
  • General notes

     This product was previously labelled as Proteasome beta 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    (without Mg2+ and Ca2+),
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 26 kDa.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similarities

    Belongs to the peptidase T1B family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ25321 antibody
    • HC5 antibody
    • KIAA1838 antibody
    • Macropain subunit C5 antibody
    • Multicatalytic endopeptidase complex subunit C5 antibody
    • Proteasome (prosome macropain) subunit beta type 1 antibody
    • Proteasome beta 1 subunit antibody
    • Proteasome component C5 antibody
    • Proteasome gamma chain antibody
    • Proteasome subunit beta type 1 antibody
    • Proteasome subunit beta type-1 antibody
    • Proteasome subunit HC5 antibody
    • PSB1_HUMAN antibody
    • PSC5 antibody
    • PSMB 1 antibody
    • PSMB1 antibody
    see all

Images

  • All lanes : Anti-PSMB1 antibody (ab196623) at 1/500 dilution

    Lane 1 : MCF7 cell extract
    Lane 2 : HL60 cell extract
    Lane 3 : SW480 cell extract
    Lane 4 : HepG2 cell extract
    Lane 5 : SW620 cell extract
    Lane 6 : K562 cell extract
    Lane 7 : PC3 cell extract
    Lane 8 : Mouse skeletal muscle cell extract
    Lane 9 : Mouse liver cell extract

    Developed using the ECL technique.

    Predicted band size: 26 kDa

  • Immunofluorscence analysis of U20S cells labeling PSMB1 with ab196623  at 1/50 dilution (top panel).

    Blue: DAPI for nuclear staining (bottom panel).

  • Immunohistochemical analysis of formalin fixed and paraffin embedded rat testis labeling PSMB1 with ab196623 at a 1/200 dilution.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded human breast cancer tissue labeling PSMB1 with ab196623 at a 1/200 dilution.

References

ab196623 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196623.
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