Key features and details
- Rabbit polyclonal to PSMB1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PSMB1 antibody
See all PSMB1 primary antibodies
DescriptionRabbit polyclonal to PSMB1
The antibody detects endogenous level of total PSMB1 protein.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant full length protein corresponding to Human PSMB1.
Database link: P20618
- MCF7, HL-60, SW480, K562, PC3, mouse skeletal muscle and mouse liver cell line extracts. Human breast cancer and rat testis tissue. U20S cells.
This product was previously labelled as Proteasome beta 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+),
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 26 kDa.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- FLJ25321 antibody
- HC5 antibody
- KIAA1838 antibody
All lanes : Anti-PSMB1 antibody (ab196623) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : HL60 cell extract
Lane 3 : SW480 cell extract
Lane 4 : HepG2 cell extract
Lane 5 : SW620 cell extract
Lane 6 : K562 cell extract
Lane 7 : PC3 cell extract
Lane 8 : Mouse skeletal muscle cell extract
Lane 9 : Mouse liver cell extract
Developed using the ECL technique.
Predicted band size: 26 kDa
Immunofluorscence analysis of U20S cells labeling PSMB1 with ab196623 at 1/50 dilution (top panel).
Blue: DAPI for nuclear staining (bottom panel).
Immunohistochemical analysis of formalin fixed and paraffin embedded rat testis labeling PSMB1 with ab196623 at a 1/200 dilution.
Immunohistochemical analysis of formalin fixed and paraffin embedded human breast cancer tissue labeling PSMB1 with ab196623 at a 1/200 dilution.
ab196623 has not yet been referenced specifically in any publications.